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Conformation and expression of the albumin gene of young and old rats

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Abstract

The conformation and expression of the albumin gene in the liver of young (21-) and old (85-week) rats were studied. Digestion of nuclei with MNase shows no differences in the nucleosomal organization in the coding region of the gene in the two ages. The gene has a DNase I hypersensitive site which is distinctly less sensitive in old rats. Its 5′-CCGG-3′ sequences are more methylated in the old in which its rate of transcription is also lower.

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Singh, A., Singh, S. & Kanungo, M.S. Conformation and expression of the albumin gene of young and old rats. Mol Biol Rep 14, 251–254 (1990). https://doi.org/10.1007/BF00429893

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Key words

  • aging
  • nucleosomal organisation
  • DH-site
  • CCGG-methylation