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Internode length in Zea mays L.

The dwarf-1 mutation controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1

Abstract

[13C, 3H]Gibberellin A20 (GA20) has been fed to seedlings of normal (tall) and dwarf-5 and dwarf-1 mutants of maize (Zea mays L.). The metabolites from these feeds were identified by combined gas chromatography-mass spectrometry. [13C, 3H]Gibberellin A20 was metabolized to [13C, 3H]GA29-catabolite and [13C, 3H]GA1 by the normal, and to [13C, 3H]GA29 and [13C, 3H]GA1 by the dwarf-5 mutant. In the dwarf-1 mutant, [13C, 3H]GA20 was metabolized to [13C, 3H]GA29 and [13C, 3H]GA29-catabolite; no evidence was found for the metabolism of [13C, 3H]GA20 to [13C, 3H]GA1. [13C, 3H]Gibberellin A8 was not found in any of the feeds. In all feeds no dilution of 13C in recovered [13C, 3H]GA20 was observed. Also in the dwarf-5 mutant, the [13C]label in the metabolites was apparently undiluted by endogenous [13C]GAs. However, dilution of the [13C]label in metabolites from [13C, 3H]GA20 was observed in normal and dwarf-1 seedlings. The results from the feeding studies provide evidence that the dwarf-1 mutation of maize blocks the conversion of GA20 to GA1.

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Abbreviations

GAn :

gibberellin An

GC-MS:

combined gas chromatography-mass spectrometry

HPLC:

high-performance liquid chromatography

RP:

reverse phase

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Spray, C., Phinney, B.O., Gaskin, P. et al. Internode length in Zea mays L.. Planta 160, 464–468 (1984). https://doi.org/10.1007/BF00429764

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Key words

  • Gibberellin metabolism
  • Mutant (dwarf, Zea)
  • Stem elongation
  • Zea (gibberellin metabolism)