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Archives of Microbiology

, Volume 148, Issue 1, pp 29–33 | Cite as

Cyclic AMP regulation of glucose transport in germinating Pilobolus longipes spores

  • J. A. Bourret
  • C. M. Smith
Original Papers

Abstract

Pilobolus longipes spores were activated by either glucose or 6-deoxyglucose. Glucose-induced spore activation was previously shown to follow an increase in intracellular cyclic AMP. Concurrent with glucose-induced spore activation, were shifts in 6-deoxyglucose transport kinetics towards higher Vmax and Km values. Cyclic AMP derivatives also caused spore activation and similar changes in the kinetic parameters of 6-deoxyglucose transport. The time course of activation was paralleled by changes in transport activity. Inhibition of phosphodiesterase alone did not cause activation or induce changes in transport activity, but in combination with sub-optimal levels of either 6-deoxyglucose or cAMP derivatives, it amplified the germination signals to produce large increases in both spore activation and 6-deoxyglucose transport activity. These results support the conclusion that glucose transport in germinating spores is regulated by cAMP.

Key words

Pilobolus Cyclic AMP Spores Glucose 6-Deoxyglucose Transport Germination Activation 

Abbreviations

IBMX

3-isobutyl-1-methylxanthine; monobutyryl cyclic AMP

N6

monobutyryladenosine 3′:5′-cyclic monophosphate

8-bromo cyclic AMP

8-bromoadenosine 3′:5′-cyclic monophosphate

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References

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • J. A. Bourret
    • 1
  • C. M. Smith
    • 1
  1. 1.Department of BiologyCalifornia State UniversityLong BeachUSA

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