The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and below this concentration the amount of HCN produced decreases sharply and at 0.1 and 100 mM phosphate low HCN production occurs. If a culture growing at 0.1 mM phosphate and producing low HCN is shifted to 10 mM phosphate, HCN biosynthesis resumes. Experiments with chloramphenicol indicate that de novo-protein synthesis is required for the process.
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Aldridge, W. N.: A new method for the estimation of micro quantities of cyanide and thiocyanate. Analyst 69, 262–265 (1944)
Brysk, M. N., Lauinger, C., Ressler, C.: Biosynthesis of cyanide from (2-14C15N) glycine in Chromobacterium violaceum. Biochim. biophys. Acta (Amst.) 184, 583–588 (1969)
Castric, P. A.: Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Canad. J. Microbiol. 21, 613–618 (1975)
Demain, A. L., Inamine, E.: Biochemistry and regulation of streptomycin and mannosidostreptomycinase (α-d-mannosidase) formation. Bact. Rev. 34, 1–19 (1970)
Hou, C. I., Gronlund, F., Campbell, J. J. R.: Influence of phosphate starvation on cultures of Pseudomonas aeruginosa. J. Bact. 92, 851–855 (1966)
Hutchinson, S. A.: Biological activities of fungal metabolites. Ann. Rev. Phytopath. 11, 223–246 (1973)
Lorck, H.: Production of hydrocyanic acid by bacteria. Physiol. Plant. 1, 142–146 (1948)
Mann, M. B., Huang, P. C.: New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low phosphate medium. J. Bact. 118, 209–212 (1974)
Meganathan, R., Castric, P. A.: Effect of inorganic phosphate on hydrogen cyanide biosynthesis by Pseudomonas aeruginosa. Abstracts of the Ann. Soc. Microbiol., p. 141 (1976)
Michaels, R., Corpe, W. A.: Cyanide formation by Chromobacterium violaceum. J. Bact. 89, 106–112 (1965)
Miller, A. V., Walker, J. B.: Accumulation of streptomycin phosphate in cultures of streptomycin producers grown an a highphosphate medium. J. Bact. 104, 8–12 (1970)
Patty, A. F.: The production of hydrocyanic acid by Bacillus pyocyaneus. J. infect. Dis. 29, 73–77 (1921)
Robbers, J. E., Robertson, L. W., Hornemann, K. M., Jundra, A., Floss, H. G.: Physiological studies on ergot: further studies on the induction of alkaloid synthesis by Tryptophan and its inhibition by phosphate. J. Bact. 112, 791–796 (1972)
Weinberg, E. D.: Secondary metabolism: Raison d'être. Persp. Biol. Med. 14, 565–577 (1971)
Weinberg, E. D.: Secondary metabolism: Control by temperature and inorganic phosphate. Develop. Industr. Microbiol. 15, 70–81 (1974)
Wissing, F.: Growth curves and pH optima for cyanide producing bacteria. Physiol. Plant. 21, 589–593 (1968)
Wissing, F.: Cyanide production from glycine by a homogenate from a Pseudomonas species. J. Bact. 121, 695–699 (1975)
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Meganathan, R., Castric, P.A. The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa . Arch. Microbiol. 114, 51–54 (1977). https://doi.org/10.1007/BF00429629
- Hydrogen cyanide biosynthesis
- Pseudomonas aeruginosa
- Phosphate effect on HCN
- Secondary metabolism