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The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa

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The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and below this concentration the amount of HCN produced decreases sharply and at 0.1 and 100 mM phosphate low HCN production occurs. If a culture growing at 0.1 mM phosphate and producing low HCN is shifted to 10 mM phosphate, HCN biosynthesis resumes. Experiments with chloramphenicol indicate that de novo-protein synthesis is required for the process.

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Meganathan, R., Castric, P.A. The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa . Arch. Microbiol. 114, 51–54 (1977). https://doi.org/10.1007/BF00429629

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Key words

  • Hydrogen cyanide biosynthesis
  • Pseudomonas aeruginosa
  • Phosphate effect on HCN
  • Secondary metabolism