Growth regulators, Rhizobium and nodulation in peas
Indole-3-acetic acid from the culture medium of nodulating and non-nodulating strains of R. leguminosarum
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Abstract
Indole-3-acetic acid (IAA) has been identified in the culture medium of nodulating and non-nodulating strains of Rhizobium lebuminosarum by gas chromatography-mass spectrometry. The levels of IAA produced by the different strains have been quantified using multiple ion monitoring and a deuterated internal standard. Indole-3-acetic acid is produced in the absence of exogenous tryptophan in all strains but its level is greatly stimulated by applied tryptophan. No correlation has been established between the ability to nodulate peas and the ability to produce IAA.
Key words
Auxin and nodulation Pisum (nodulation) Plasmid Rhizobium Root noduleAbbreviations
- GCMS
gas chromatography-mass spectrometry
- HPLC
high performance liquid chromatography
- IAA
indole-3-acetic acid
- MIM
multiple ion monitoring
- TMS
trimethylsilyl
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References
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