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Archives of Microbiology

, Volume 134, Issue 1, pp 28–32 | Cite as

Isolation and characterization of two new methane-producing cocci: Methanogenium olentangi, sp. nov., and Methanococcus deltae, sp. nov.

  • R. E. Corder
  • L. A. Hook
  • J. M. Larkin
  • J. I. Frea
Original Papers

Abstract

Two new methane-producing cocci have been isolated from separate sediment enrichments using a defined synthetic growth medium. A freshwater methanogen, Methanogenium olentangyi, sp. nov., was isolated from Olentangy River sediment, Columbus, Ohio. The organism was a non-motile, irregular coccus that produced methane from CO2 plus H2, and required acetate for growth. A marine methanogen, Methanococcus deltae, sp. nov., was isolated from Gulf of Mexico sediments and was a non-motile, irregular coccus that grew on CO2 plus H2, or on formate. The guanine-plus-cytosine content of the deoxyribonucleic acid of the isolates was 54.4 and 40.5 mol%, respectively.

Key words

Methanogenium olentangyi Methanococcus deltae Methane-producing cocci 

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References

  1. Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296Google Scholar
  2. Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791Google Scholar
  3. Conway de Macario E, Macario AJL, Wolin MJ (1982) Specific antisera and immunological procedures for characterization of methanogenic bacteria. J Bacteriol 149:320–328Google Scholar
  4. Conway de Macario E, Wolin MJ, Macario AJL (1982a) Antibody analysis of relationships among methanogenic bacteria. J Bacteriol 149:316–319Google Scholar
  5. Conway de Macario E, Wolin MJ, Macario AJL (1981) Immunology of Archaebacteria that produce methane gas. Science 214:74–75Google Scholar
  6. Corder RE, Hook LA, Frea JI (1982) Isolation and characterization of two new methane producing cocci. Abstr Amer Soc Microbiol, Washington, D. C., p 111Google Scholar
  7. DeLey J, Frateur J (1974) Genus Acetobacter Beijerinck 1898. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology 8th ed. Williams and Wilkins Co, Baltimore, MD, pp 276–278Google Scholar
  8. Ferguson TJ, Mah RA (1982) Isolation and characterization of a formate and H2/CO2 utilizing thermophilic methanogen. Abstr Amer Soc Microbiol, Washington, D.C., p 110Google Scholar
  9. Fox GE, Magrum LJ, Balch WE, Wolfe RS, Woese CR (1977) Classification of methanogenic bacteria by 16S ribosomal RNA characterization. Proc Natl Acad Sci USA 74:4537–4541Google Scholar
  10. Gibson T, Gordon RE (1974) Gerus I. Bacillus Cohn 1872. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins Co., Baltimore, MD, pp 529–550Google Scholar
  11. Holdeman LV, Moore WEC (1974) Genus I. Bacteriodes Castillani and Chalmers 1919. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins Co., Baltimore, MD, pp 385–404Google Scholar
  12. Jones WJ, Paynter MJB (1981) Characterization of Methanogenium paludismaritimae sp. nov., a marine methanogen. Abstr Amer Soc Microbiol, Washington, D.C., p 111Google Scholar
  13. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218Google Scholar
  14. Rivard CJ, Smith PH (1982) Isolation and characterization of a thermophilic marine methanogen Methanogenium thermophilicum sp. n.. Abstr Amer Soc Microbiol, Washington, D.C., p 111Google Scholar
  15. Romesser JA, Wolfe RS, Mayer F, Spiess E, Walther-Mauruschat A (1979) Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch Microbiol 121:147–153Google Scholar
  16. Schildkrant CL, Marmur J, Doty P (1962) Determination of base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol 4:430–443Google Scholar
  17. Stadtman TC, Barker HA (1951) Studies on methane fermentation.X. A new formate-decomposing bacterium, Methanococcus vannielli. J Bacteriol 62:269–280Google Scholar
  18. Whitman WB, Ankwanda E, Wolfe RS (1982) Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol 149:852–863Google Scholar
  19. Zeikus JG (1977) The biology of methanogenic bacteria. Bacteriol Rev 41:514–541Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • R. E. Corder
    • 1
  • L. A. Hook
    • 1
  • J. M. Larkin
    • 2
  • J. I. Frea
    • 1
  1. 1.Department of MicrobiologyThe Ohio State UniversityColumbusUSA
  2. 2.Department of MicrobiologyLouisiana State UniversityBaton RougeUSA

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