Glucose-6-phosphate dehydrogenase (d-glucose-6-phosphate: NADP+ l-oxidoreductase EC 18.104.22.168) isolated from Paracoccus denitrificans grown on glucose/nitrate exhibits both NAD+-and NADP+-linked activities. Both activities have a pH optimum of pH 9.6 (Glycine/NaOH buffer) and neither demonstrates a Mg2+ requirement. Kinetics for both NAD(P)+ and glucose-6-phosphate were investigated. Phosphoenolpyruvate inhibits both activities in a competitive manner with respect to glucose-6-phosphate. ATP inhibits the NAD+-linked activity competitively with respect to glucose-6-phosphate but has no effect on the NADP+-linked activity. Neither of the two activities are inhibited by 100 μM NADH but both are inhibited by NADPH. The NAD+-linked activity is far more sensitive to inhibition by NADPH than the NADP+-linked activity.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bailey, L. J. (ed.): Techniques in protein chemistry, p. 293. Amsterdam: Elsevier 1962
Blackkolb, F., Schlegel, H. G.: Regulation der Glucose-6-phosphat-Dehydrogenase aus Hydrogenomonas H16 durch ATP und NADH2. Arch. Mikrobiol. 63, 177–196 (1968)
Collins, V. G.: Isolation, cultivation and maintenance of autotrophs. In: Methods in microbiology, Vol. 3B (J. R. Norris, D. W. Ribbons, eds.), p. 36. New York-London: Academic Press 1969
Forget, P.: Recherches sur le métabolisme du glucose chez les bactéries dénitrifiantes. 2. Micrococcus denitrificans. Ann. Inst. Pasteur 115, 332–342 (1968)
Olive, C., Levy, H. R.: The preparation and some properties of crystalline glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Biochemistry 6, 730–736 (1967)
Slabas, A. R., Watley, F. R.: Regulation of pyruvate kinase in Micrococcus denitrificans. Biochem. Soc. Transact. 2, 929–930 (1974)
Tabita, R., Lundgren, D. G.: Glucose-6-phosphate dehydrogenase from the chemolithotroph Thiobacillus ferrooxidans. J. Bact. 108, 343–352 (1971)
Tunail, N., Schlegel, H. G.: Phosphoenolpyruvate, a new inhibitor of glucose-6-phosphate dehydrogenase. Biochem. biophys. Res. Commun. 49, 1554–1560 (1972)
About this article
Cite this article
Slabas, A.R., Wahtley, F.R. Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrificans grown on glucose/nitrate. Arch. Microbiol. 112, 225–227 (1977). https://doi.org/10.1007/BF00429339
- Glucose-6-phosphate dehydrogenase
- Paracoccus denitrificans
- Nicotinamide adenine dinucleotide (phosphate)
- Enzyme regulation