Archives of Microbiology

, Volume 118, Issue 3, pp 317–319 | Cite as

The insensitivity of mushroom nuclear RNA polymerase activity to inhibiton by amatoxins

  • Paul A. Horgen
  • Allan C. Vaisius
  • Joseph F. Ammirati
Short Communication

Abstract

Nuclear fractions isolated from Amanita phalloides, Amanita muscaria and Agaricus bisporus were subjected to in vitro RNA synthesis assays in the presence of various concentrations of amatoxins. The mushroom nuclei were highly insensitive to inhibition by amatoxin when compared to assays of nuclear fractions isolated from the Oömycete fungus, Achlya ambisexualis and from rabbit brain.

Key words

RNA polymerase Transcription Amatoxins Nuclei Poisonous mushrooms In vitro RNA synthesis Agricus bisporus Amanita phalloides Amanita muscaria Achlya ambisexualis 

Abbreviations

DTT

dithiothreitol

PMSF

phenylmethyl sulfonyl fluoride

MES

2[N-morpholino] ethane sulfonic acid

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Paul A. Horgen
    • 1
    • 2
  • Allan C. Vaisius
    • 1
    • 2
  • Joseph F. Ammirati
    • 1
    • 2
  1. 1.North American Poison Mushroom Research CentreUniversity of TorontoMississaugaCanada
  2. 2.Department of BotanyUniversity of TorontoMississaugaCanada

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