Enzymatic adaptations to treadmill training in skeletal muscle of young and old rats

  • Jorge Sanchez
  • Catherine Bastien
  • Hugues Monod


The activities of lactic dehydrogenase (LDH), hexokinase (HK), citrate synthetase (CS), and 3-hydroacyl CoA dehydrogenase (HAD), were measured in Soleus and extensor digitorum longus (EDL) muscles of four different age groups of male Wistar rats. Young rats (3 months) and old rats (24 months) were trained during 12 weeks by a 3 days a week running exercises session. Each training session was of 2 h. All the enzymatic activities increased between 3 and 6 months in both Soleus and EDL muscles. During aging (6–27 months) Soleus showed about the same percent decrease in enzymatic activities (30%) for the four enzymes studied. In contrast with Soleus, CS activity of EDL increased with age but HK, LDH, and HAD decreased. The training induced an improvement of all enzymatic activities, except for LDH, which decreased with training in same cases. In the old group the training effect was more important than in the young group. When the relative enzymatic activities were compared it was found that Soleus muscle keeps its energy supply enzyme pattern during aging, whereas EDL evolutes to a more oxidative metabolism. Moreover old skeletal muscle remains trainable and training induces an enzymatic adaptation which follows the same pattern in both muscles: an increase of HK, CS, and HAD activities and a decrease of LDH.

Key words

Rats Training Skeletal muscle Aging Enzymes of energetic metabolism 


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  1. Baldwin KM, Winder WW, Terjung RL, Holloszy JO (1973) Glycolytic enzymes in different types of skeletal muscle: adaptation to exercise. Am J Physiol 1225: 962–966Google Scholar
  2. Bass A, Brdiczka D, Eyer P, Hofer S, Pette D (1969) Metabolic differentiation of distinct muscle types at the level of enzymatic organization. Eur J Biochem 10: 198–206PubMedCrossRefGoogle Scholar
  3. Bass A, Gutmann E, Hanzlikova U (1975) Biochemical and histochemical changes in energy supply-enzyme pattern of muscles of the rat during old age. Gerontologia 21: 31–46PubMedCrossRefGoogle Scholar
  4. Belcastro AN, Wenger H (1982) Myofibril and sarcoplasm reticulum changes with exercise and growth. Eur J Appl Physiol 49: 87–59CrossRefGoogle Scholar
  5. Belcastro AN, Wenger H, Nikei T, Secord D, Bonen A (1980) Functional overload of rat fast twitch skeletal muscle during development. J Appl Physiol Respir Environ Exerc Physiol 49: 583–588Google Scholar
  6. Crabtree B, Newsholme EA (1972) The activities of phosphorylase, hexokinase, phosphofructokinase, lactate dehydrogenase, and the glycerol-3-phosphate dehydrogenase in muscles from vertebrates and invertebrates. Biochem J 126: 49–58PubMedGoogle Scholar
  7. Goodrick CL (1980) Effects of long-term voluntary wheel exercise on male and female Wistar rats. I Longevity, Body Weigth, and metabolic rate. Gerontology 26: 22–33PubMedCrossRefGoogle Scholar
  8. Gutmann E, Hanzlikova V (1975) Changes in neuromuscular relationships in aging. In: Ordy JM, Brizzee KR (eds) Neurobiology of aging. Plenum Press, New York London, pp 193–207Google Scholar
  9. Harri MNE, Narvola I (1979) Physical training under the influence of beta blockade in rats: Effects on adrenergic responses. Eur J Appl Physiol 41: 199–210CrossRefGoogle Scholar
  10. Holloszy JO, Booth FW (1976) Biochemical adaptations to endurance exercise in muscle. Ann Rev Physiol 38: 273–291CrossRefGoogle Scholar
  11. Holloszy JO, Oscai LB, Don IJ, Molé PA (1971) Mitochondrial citric acid cycle related enzymes: adaptative response to exercise. Biochem Biophys Res Commun 40: 1368–1373Google Scholar
  12. Kaldor G, DiBattista WJ (1978) Aging in muscle. Raven Press New YorkGoogle Scholar
  13. Karlsson J, Sjödin B, Thorstensson A, Hultén B, Frith K (1975) LDH isozymes in skeletal muscles of endurance and strength trained athletes. Acta Physiol Scand 93: 150–156PubMedGoogle Scholar
  14. Kiessling KH, Pilström L, Bylund AC, Saltin B, Piehl K (1974) Enzyme activities and morphometry in skeletal muscle of middle-aged men after training. Scand J Clin Lab Invest 33: 63–69PubMedGoogle Scholar
  15. Larsson L, Sjödin B, Karlsson J (1978) Histochemical and biochemical changes in human skeletal muscle with age in sedentary males, age 22–65 years. Acta Physiol Scand 103: 31–39PubMedGoogle Scholar
  16. Lowry OH, Passonneau JV (1973) A flexible system of enzymatic analysis. Academic Press, New YorkGoogle Scholar
  17. Morgan TE, Cobb LA, Short FA, Ross R, Gunn DR (1971) Effects of long-term exercise on human muscle. In: Pernow B, Saltin B (eds) Muscle metabolism during exercise. Plenum Press, New York London, pp 87–95Google Scholar
  18. Nygaard E, Sánchez J (1982) Intramuscular variation of fiber types in the brachial biceps and the lateral vastus muscles of elderly men: How representative is a small biopsy sample? Anat Rec 203: 451–459PubMedCrossRefGoogle Scholar
  19. örlander J, Aniansson A (1980) Effects of physical training on skeletal muscle metabolism and ultrastructure in 70 to 75-years-old men. Acta Physiol Scand 109: 149–154PubMedCrossRefGoogle Scholar
  20. örlander J, Kiessling KH, Ekblom B (1980) Time course of adaptation to low intensity training in sedentary men: dissociation of central and local effects. Acta Physiol Scand 108: 85–90PubMedGoogle Scholar
  21. Pette D (1980) Plasticity of Muscle. Walter de Gruyter, Berlin New YorkGoogle Scholar
  22. Roch-Norlund AE, Borrebaek B (1978) The decrease with age in the activities of enzymes of human skeletal muscle. Some observations on Palmityl-carnitine formation hexokinase activity, and lactate dehydrogenase activity. Biochem Med 20: 378–381CrossRefGoogle Scholar
  23. Skinner JS, Tipton CH, Vailas AC (1982) Exercise, physical training and the ageing process. In: Vüdik A (ed) On biology of ageing. Academic Press, London, pp 407–408Google Scholar
  24. Staudte HW, Pette D (1972) Correlation between enzymes of energy-supplying metabolism as a basic pattern of organization in muscle. Comp Biochem Physiol 416: 533–540Google Scholar
  25. Steinhagen-Thiessen E, Reznik A, Hilz H (1980) Negative adaptation to physical training in senile mice. Mech Ageing Dev 12: 231–236PubMedCrossRefGoogle Scholar
  26. Suominen H, Heikikinen E (1975) Enzyme activities in muscle and connective tissue of m. vastus lateralis in habitually training and sedentary 33 to 70-years-old-men. Eur J Appl Physiol 34: 249–254CrossRefGoogle Scholar
  27. Thomas TR, Londeree BR, Gerhardt K, Gehrke CW (1978) Fatty acid pattern and cholesterol in skeletal muscle of men aged 22 to 73. Mech Ageing Dev 8: 429–434PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Jorge Sanchez
    • 1
  • Catherine Bastien
    • 1
  • Hugues Monod
    • 1
  1. 1.Laboratoire de Physiologie du Travail C.N.R.S.Paris Cedex 13France

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