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Marking of a Sym plasmid of Rhizobium with Tn7

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Summary

Transposon Tn7 was inserted into wide host range plasmid pSUP202 and used as a suicide plasmid vehicle for transposon mutagenesis in Rhizobium leguminosarum. Tn7 is transposed with high frequency into the self-transmissible plasmid pJB5JI without affecting the transfer, nodulation and nitrogen fixation functions. Tn7 transposition provides a useful tool for marking symbiotic plasmids.

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Correspondence to Gerhard Miksch.

Additional information

Communicated by H.Böhme

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Miksch, G., Lentzsch, P. Marking of a Sym plasmid of Rhizobium with Tn7 . Mol Gen Genet 206, 510–514 (1987). https://doi.org/10.1007/BF00428893

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Key words

  • Transposon
  • Rhizobium
  • Sym plasmid