Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Transposon-like structures in the Bacteriodes fragilis MLS plasmid pIP 410

  • 24 Accesses

  • 24 Citations

Summary

Homoduplexes of plasmid plP 410 encoding MLS resistance in Bacteroides fragilis strain 92 revealed two “transposon-like” structures:a 17.4 kb loop flanked by 0.75 inverted repeats, and a 4.5 kb loop flanked by 0.2 kb inverted repeats. The large structure is deleted in a derivative plasmid harboured by a MLS susceptible strain. We have also showed that the long and short inverted repcats are structurally related.

This is a preview of subscription content, log in to check access.

References

  1. Bostein D, Kleckner N (1977) Translocation and illegitimate recombination by the tetracycline resistance element Tn 10. In: Bukhari AI, Shapiro JA, Adhya SL (eds) Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 185–203

  2. Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 21D:413–428

  3. Fiers W, Contreras R, Haegeman G, Rogiers R, Van de Voorde A, Van Heuverswyn H, Van Herreweghe J, Volckaert G, Ysebaert M (1978) Complete nucleotide sequence of SV 40 DNA. Nature 273:113–120

  4. Kleckner N (1977) Translocatable elements in procaryotes. Cell 11:11–23

  5. Privitera G, Dublanchet A, Sebald M (1979a) Transfer of multiple antibiotic resistance between subspecies of Bacterioides fragilis. J Infect Dis 139:97–101

  6. Privitera G, Sebald M, Fayolle F (1979b) Common regulatory mechanism of expression and conjugative ability of a tetracycline resistance plasmid in Bacteriodes fragilis. Nature 278:657–659

  7. Sanger F, Air GM, Barrell BG Brown NL, Coulson AR, Fiddes JC, Hutchinson CV III, Slocombe PM, Smith M (1977) Nucleotide sequence of bacteriophage Φx174 DNA. Nature 265:687–695

  8. Starlinger P (1978) DNA rearrangements in procaryotes. Ann Rev Genet 11:103–126

  9. Tally FP, Snydman DR, Gorbach SL, Malamy MH (1979) Plasmidmediated transferable clindamycin and erythromycin resistance in Bacteroides fragilis. J Infect Dis 139:83–88

  10. Welch RA, Kevin JR, Macrina L (1979) Transferable macrolide-lincosamide resistance in Bacteroides. Plasmid 2:261–268

  11. Welch RA, Macrina FL (1980) Deletion analysis of a transferable Bacteroides R-plasmid. In: Stuttard C, Rozee KR (eds) Plasmids and transposons. Environmental effects and maintenance mechanism. Academic Press, pp 137–144

Download references

Author information

Correspondence to Michel Magot.

Additional information

Communicated by H. Saedler

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Magot, M., Fayolle, F., Privitera, G. et al. Transposon-like structures in the Bacteriodes fragilis MLS plasmid pIP 410. Molec. Gen. Genet. 181, 559–561 (1981). https://doi.org/10.1007/BF00428754

Download citation

Keywords

  • Inverted Repeat
  • Large Structure
  • Susceptible Strain
  • Derivative Plasmid