Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1

Summary

Transducing λ phages have been isolated that carry segments of the Escherichia coli chromosome in the aspC region, 20.5 min on the E. coli map. One of these phages λaspC2, carries rpsA, the structural gene for the ribosomal protein S1.

A three kilobase fragment from this phage, cloned into either the plasmid pACYC184 or the plasmid pBR322, was found to express S1.

In cells carrying the rpsA gene on the high copy number plasmid pBR322 the rate of rpsA mRNA synthesis was increased 40-fold, whereas the rate of protein S1 synthesis was doubled, in comparison with these rates in an rpsA haploid.

This is a preview of subscription content, log in to check access.

References

  1. Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12. Microbiol Reviews 6:1–56

  2. Bremer H, Yuan D (1968) RNA chain growth rate in Escherichia coli. J Mol Biol 38:163–180

  3. Clark DJ, Maaløe O (1967) DNA replication and the division cycle in Escherichia coli. J Mol Biol 23:99–112

  4. Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106

  5. Dahlberg AE, Dahlberg JE (1975) Binding of ribosomal protein S1 of Escherichia coli to the 3′ end of 16S rRNA. Proc Natl Acad Sci USA 72:2940–2944

  6. Dennis PP, Fiil NP (1979) Transcriptional and postranscriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColEI plasmids in the bacterium Escherichia coli. J Biol Chem 254:7540–7547

  7. Draper DE, von Hippel PH (1978) Nucleic acid binding properties of Escherichia coli ribosomal protein, S1. I. Structure and interactions of binding site I. J Mol Biol 122:321–338

  8. Duin J van, van Knippenberg PH (1974) Functional heterogeneity of the 30 S ribosomal subunit of Escherichia coli III. Requirement of protein S1 for translation.J Mol Biol 84:185–195

  9. Duin J van, Kurland CG (1970) Functional heterogeneity of the 30S ribosomal subunit of E. coli. Mol Gen Genet 109:169–176

  10. Gausing K (1977) Regulation of ribosome production in Escherichia coli: Synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates. J Mol Biol 115:335–354

  11. Gelfand DH, Rudo N (1977) Mapping of the aspartate and aromatic amino acid aminotransferase genes tyrB and aspC. J Bacteriol 130:441–444

  12. Gelfand DH, Steinberg RA (1977) Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J Beteriol 130:429–440

  13. Held WA, Mizushima S, Nomura M (1973) Reconstitution of Escherichia coli 30S ribosomal subunits from purified molecular components. J Biol Chem 248:5720–5730

  14. Kitakawa M, Blumenthal L, Isono K (1980) Isolation and characterization of specialized transducing λ phages carrying ribosomal protein genes of Escherichia coli. Mol Gen Genet 180:343–349

  15. Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor, Laboratory, New York

  16. O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

  17. Ono M, Kuwano M, Mizushima S (1979) Genetic analysis of a mutation affecting ribosomal protein S1 in Escherichia coli. Mol Gen Genet 174:11–15

  18. Pedersen S, Bloch PL, Reeh S, Neidhardt FC (1978) Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell, 14:179–190

  19. Pedersen S, Reeh SV, Parker J, Watson RJ, Friesen JD, Fiil NP (1976) Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages λdrif d and λdfus-3 Mol Gen Genet 144:339–343

  20. Reeh S, Pedersen S, Friesen JD (1976) Biosynthetic regulation of individual proteins in relA + and relA strains of Escherichia coli. Mol Gen Genet 149:279–289

  21. Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693

  22. Schrenk WJ, Weisberg RA (1975) A simple method for making new transducing lines of coliphage λ. Mol Gen Genet 137:101–107

  23. Yates JL, Arfsten AE, Nomura M (1980) In vitro expression of Escherichia coli ribosomal protein genes: Autogenous inhibition of translation. Proc Natl Acad Sci USA 77:1837–1841

Download references

Author information

Additional information

Communicated by K. Isono

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Christiansen, L., Pedersen, S. Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1. Molec. Gen. Genet. 181, 548–551 (1981). https://doi.org/10.1007/BF00428751

Download citation

Keywords

  • Restriction Endonuclease
  • Structural Gene
  • Ribosomal Protein
  • High Copy Number
  • mRNA Synthesis