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Exfoliation of respiratory epithelium in hamster tracheal organ cultures infected with Mycoplasma pneumoniae

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Summary

Hamster tracheal organ cultures were infected with M. pneumoniae and examined sequentially by transmission and scanning electron microscopy to correlate surface with intracellular alterations. Infection was established by culture and the demonstration of morphologically compatible organisms on the mucosal surface. Ciliated epithelial cells developed vacuolization of the apical and subnuclear cytoplasm and eventually fragmented along planes formed by coalescing vacuoles. Non-ciliated cells showed apical swelling and loss of microvilli during the course of infection. After degeneration and sloughing of both ciliated and non-ciliated cells, a flattened layer of intact basal cells covered the submucosa. It is likely that progressive vacuolization of epithelial cells leads to exfoliation of both cells and cell fragments in M. pneumoniae infection. Since organisms frequently are associated with these exfoliated cells, their potential presence in sputum and lavage specimens could prove to be of diagnostic importance.

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References

  1. Asmundsson T, Kilburn KH, McKenzie WN (1973) Injury and metaplasia of airway cells due to SO2. Lab Invest 29:41–53

  2. Basrur PK, Basrur VR (1976) Respiratory surface epithelium: Scanning electron microscopic technique for bioassay on inhalants. In: Scanning Electron Microscopy. IITRI 1976, Chicago, p 623

  3. Biberfeld G, Biberfeld P (1970) Ultrastructural features of M. pneumoniae. J Bacteriol 102:855–861

  4. Boatman ES, Sato S, Frank R (1974) Acute effects of ozone on cat lungs. Am Rev Respir Dis 110:157–169

  5. Chanock RM (1965) Mycoplasma infections in man. N Engl J Med 273:1199–1206

  6. Clyde WA (1973) Models of M. pneumoniae infection. J Infect Dis 127:S69–72

  7. Collier AM (1972) Pathogenesis of M. pneumoniae infection as studied by human foetal trachea in organ culture. In: Pathogenic Mycoplasmas, Assoc Scientific Pub, Amsterdam, p 307

  8. Collier AM, Baseman JB (1973) Organ culture techniques with mycoplasmas. Ann NY Acad Sci 225:277–289

  9. Collier AM, Clyde WA (1971) Relationship between M. pneumoniae and human respiratory epithelium. Infec Immun 3:694–701

  10. Collier AM, Clyde WA (1974) Appearance of M. pneumoniae in lungs of experimentally infected hamsters and sputa from patients with natural disease. Am Rev Respir Dis 110:765–773

  11. Collier AM, Clyde WA, Denny FW (1969) Biologic effects of M. pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proc Soc Exp Biol Med 132:1153–1158

  12. Collier AM, Clyde WA, Denny FW (1971) M. pneumoniae in hamster tracheal organ culture: Immunofluorescent and electron microscopic studies. Proc Soc Exp Biol Med 136:569–573

  13. Craighead JE (1975) Report of a workshop: Disease accentuation after immunization with inactivated microbial vaccines. J Infect Dis 131:749–753

  14. Craighead JE (1968) Cytopathic effects of parainfluenza type 3 in organ cultures of human respiratory tract tissue. Am J Pathol 52:287–300

  15. Dahlgren SE, Dalen H (1972) Ultrastructural observations of chemically induced inflammation in guinea pig trachea. Virchows Archiv [Cell Pathol] 11:211–223

  16. Dejani AS, Clyde WA, Denny FW (1965) Experimental infection with M. pneumoniae (Eaton's agent). J Exp Med 121:1071–1086

  17. Denny FW, Clyde WA, Glezen WP (1971) Mycoplasma pneumoniae disease: Clinical spectrum, pathophysiology, epidemiology and control. J Infect Dis 123:74–92

  18. Fernald GW (1969) Aspects of experimental M. pneumoniae infection. J Infect Dis 119:255–266

  19. Fernald GW (1972) In vitro response of human lymphocytes to Mycoplasma pneumoniae. Infec Immun 5:552–558

  20. Fernald GW (1973) Role of host response in M. pneumoniae disease. J Infect Dis 127:55–58

  21. Fernald GW, Glezen WP (1973) Humoral and cellular immune responses to an inactivated Mycoplasma pneumoniae vaccine in children. J Infect Dis 127:498–504

  22. Hara K, Izumikawa K, Kinoshita I, Ota M, Ikebe A, Koike M, Hamada M (1974) Experimental infection with M. pneumoniae in young hamster: Location of ferritin-labeled antibody binding to infective tissue. Tohoku J Exp Med 114:315–337

  23. Harris CC, Sporn MG, Kaufman DG, Smith JM, Baker MS, Saffiotti U (1971) Acute ultrastructural effects of benzo (a) pyrene and ferric oxide on the hamster tracheobronchial epithelium. Cancer Res 31:1977–1989

  24. Hayflick L (1965) Tissue cultures and mycoplasmas. Tex Rep Biol Med 23:S285–303

  25. Hu PC, Collier AM, Baseman JB (1975) Alterations in the metabolism of hamster tracheas in organ culture after infection with virulent M. pneumoniae. Infec Immun 11:704–710

  26. Lippman RP, Clyde WA, Denny FW (1969) Characteristics of virulent, attenuated and avirulent M. pneumoniae strains. J Bacteriol 100:1037–1043

  27. Mossman BT, Adler KB, Craighead JE (1978) Interaction of carbon particulates with tracheal epithelium in organ culture. Environ Res 16:110–122

  28. Mossman BT, Craighead JE (1974) Topical application of polycyclic hydrocarbons to differentiated respiratory epithelium in organ culture. In: Experimental Lung Cancer. Carcinogenesis and Bio-Assays, Springer, Berlin, p 514

  29. Mossman BT, Craighead JE (1975) Long-term maintenance of differentiated respiratory epithelium in organ cultures. I. Medium composition. Proc Soc Exp Biol Med 149:227–233

  30. Mossman BT, Kessler JB, Ley BW, Craighead JE (1977) Interaction of crocidolite asbestos with hamster respiratory mucosa in organ culture. Lab Invest 36:131–139

  31. Muse KE, Collier AM, Baseman JB (1977) Scanning electron microscopic study of hamster tracheal organ cultures infected with Bordetella pertussis. J Infect Dis 136:768–777

  32. Muse KE, Dwight AP, Collier AM (1976) M. pneumoniae in hamster tracheal organ culture studied by scanning electron microscopy. Infec Immun 13:229–237

  33. Papanicolau GN (1956) Degenerative changes in ciliated cells exfoliating from bronchial epithelium as a cytologic criterion in diagnosis of diseases of the lung. NY State J Med 56:2647–2650

  34. Powell DA, Hu PC, Wilson M, Collier AM, Baseman JB (1976) Attachment of M. pneumoniae to respiratory epithelium. Infect Immun 13:959–966

  35. Reed SE, Boyde A (1972) Organ cultures of respiratory epithelium infected with rhinovirus or parainfluenza virus studied by a scanning electron microscope. Infec Immun 6:68–76

  36. Smith CB, Friedewald WT, Chanock RM (1967) Inactivated Mycoplasma pneumoniae vaccine: evaluation in volunteers. JAMA 199:353–358

  37. Wilson MH, Collier AM (1976) Ultrastructural study of Mycoplasma pneumoniae in organ culture. J Bacteriol 125:332–339

  38. Woodruff KH (1973) Ultrastructural changes in hamster tracheal ring cultures exposed to M. pneumoniae. Am J Pathol 72:91–102

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Correspondence to John E. Craighead M.D..

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Murphy, G.F., Brody, A.R. & Craighead, J.E. Exfoliation of respiratory epithelium in hamster tracheal organ cultures infected with Mycoplasma pneumoniae . Virchows Arch. A Path. Anat. and Histol. 389, 93–102 (1980). https://doi.org/10.1007/BF00428670

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Key words

  • Mycoplasma pneumoniae
  • Tracheal epithelium
  • Organ culture
  • Transmission and scanning electron microscopy