Archives of Microbiology

, Volume 113, Issue 1–2, pp 121–130 | Cite as

Ophiocordin, an antifungal antibiotic of Cordyceps ophioglossoides

  • Helmut Kneifel
  • Wilfried A. Konig
  • Wolfgang Loeffler
  • Renate Müller
Article

Abstract

An unknown antibiotic, ophiocordin, C21H22N2O8, MW: 430, was isolated from submerged cultures of Cordyceps ophioglossoides, strain TÜ 276, grown in a glycerol soybean meal medium at 27°C. The antibiotic was extracted from acidified culture fluids with n-butanol and purified by subsequent column chromatography on DEAE-Sephadex and cellulose. Studies including nuclear magnetic resonance and mass spectrometry resulted in proposals of partial structures of the molecule. Inhibition by ophiocordin could be demonstrated for a small number of fungi belonging to different taxonomic groups. Bacteria were not inhibited. The antifungal effect was antagonized by ammonia and nitrate ions and by certain amino acids.

Key words

Ophiocordin Antifungal antibiotic Cordyceps ophioglossoides Culture conditions Isolation Analytical data Partial structure Biological properties 

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Helmut Kneifel
    • 1
  • Wilfried A. Konig
    • 1
  • Wolfgang Loeffler
    • 1
  • Renate Müller
    • 1
  1. 1.Lehrstuhl Mikrobiologie IInstitut für Biologie II, der Universität TübingenTübingen 1Federal Republic of Germany
  2. 2.Abteilung für Algenforschung und AlgentechnologieGesellschaft für Strahlen-und UmweltforschungDortmundFederal Republic of Germany
  3. 3.Institut für Organische Chemie und BiochemieUniversität HamburgHamburg 13Federal Republic of Germany
  4. 4.Lenrstuhl für Mikrobiologie der Technischen UniversitätHannoverFederal Republic of Germany

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