Archives of Microbiology

, Volume 127, Issue 2, pp 167–169 | Cite as

Analysis of the fermentation pathways of clostridia using double labelled glutamade

  • Wolfgang Buckel
Short Communications


l-(4-14C, 3-3H)Glutamate was used as a tool to elucidate the pathway of its fermentation. The methylaspartate pathway was found in strains of Clostridium tetanomorphum, C. malenominatum, C. limosum, C. lentoputrescens and C. tetani, whereas the hydroxyglutarate pathway was detected in C. sporosphaeroides. These data were supported by measurement of enzyme activities present in cell-free extracts.

Key words

Clostridia l-4-14C, 3-3H)Glutamate Butyrate n-Propylamine Methylaspartate and hydroxyglutarate pathways 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Wolfgang Buckel
    • 1
  1. 1.Biochemie IFakultät für Biologie und Vorklinische Medizin der Universität RegensburgRegensburgFederal Republic of Germny

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