A method for the regulation of microbial population density during continuous culture at high growth rates
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A method for the continuous culture of microorganisms is described which employs growth-dependent pH changes to control the rate of addition of fresh medium to a culture vessel. The apparatus (the “phauxostat”) supports, at constant pH, longterm continuous culture at rates near or at the maximum of which the organisms are capable. The buffering capacity of the inflowing medium determines the steady-state population density of the culture, but the rate of growth is independent of the buffering capacity. The fundamental theory of operation is tested and some basic parameters of growth are estimated using Escherichia coli B growing continuously in media containing glucose, glycerol or DL-lactate.
Key wordsEscherichia coli B Continuous culture “Phauxostat” Growth rate
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- Anderson, P. A.: Automatic recording of the growth rates of continuously cultured microorganisms. J. gen. Physiol. 36, 733–737 (1953)Google Scholar
- Anderson, P. A.: Continuous recording of the growth of micro-organisms under turbidostatic and chemostatic control. Rev. Scient. Instrum. 27, 48–51 (1956)Google Scholar
- Bryson, V., Szybalski, W.: Microbial selection. Science 116, 45–51 (1952)Google Scholar
- Hempfling, W. P.: Repression of oxidative phosphorylation in Escherichia coli B by growth in glucose and other carbohydrates. Biochem. biophys. Res. Commun. 41, 9–15 (1970)Google Scholar
- Hempfling, W. P., Mainzer, S. E.: Effects of varying the carbon source limiting growth on yield and maintenance characteristics of Escherichia coli B in continuous culture. J. Bact. 123, 1076–1087 (1975)Google Scholar
- Herbert, D.: Some principles of continuous culture. In: Recent progress in microbiology, G. Tunevall, ed., pp. 381–396. Springfield, Ill.: Thomas 1958Google Scholar
- Herbert, D., Ellsworth, R., Telling, R. D.: The continuous culture of bacteria: A theoretical and experimental study. J. gen. Microbiol. 14, 601–622 (1956)Google Scholar
- Koch, A. L.: Turbidity measurements of bacterial cultures in some available commercial instruments. Analyt. Biochem. 38, 252–259 (1970)Google Scholar
- Northrop, J. H.: Apparatus for maintaining bacterial cultures in the steady state. J. gen. Physiol. 38, 105–115 (1954)Google Scholar
- Watson, T. G.: Steady-state operation of a continuous culture at maximum growth rate by control of carbon dioxide production. J. gen. Microbiol. 59, 83–89 (1969)Google Scholar