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Archives of Microbiology

, Volume 115, Issue 1, pp 103–108 | Cite as

Regulation of symbiotic nitrogen fixation in root nodules of alfalfa (Medicago sativa) infected with Rhizobium meliloti

  • Werner Kamberger
Article

Abstract

Symbiotic nitrogen fixation of Rhizobium meliloti bacteroids in Medicago sativa root nodules was suppressed by several inorganic nitrogen sources. Amino acids like glutamine, glutamic acid and aspartic acid, which can serve as sole nitrogen sources for the unnodulated plant did not influence nitrogenase activity of effective nodules, even at high concentrations.

Ammonia and nitrate suppressed symbiotic nitrogen fixation in vivo only at concentrations much higher than those needed for suppression of nitrogenase activity in free living nitrogen fixing bacteria. The kinetics of suppression were slow compared with that of free living nitrogen fixing bacteria. On the other hand, nitrite, which acts as a direct inhibitor of nitrogenase, suppressed very quickly and at low concentrations. Glutamic acid and glutamine enhanced the effect of ammonia dramatically, while the suppression by nitrate was enhanced only slightly.

Key words

Root nodule symbiosis Rhizobium meliloti Medicago sativa Nitrogenase activity Regulation 

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References

  1. Brown, C. M., Dilworth, M. J.: Ammonia assimilation by Rhizobium cultures and bacteriods. J. gen. Microbiol. 86, 39–48 (1975)Google Scholar
  2. Copeland, R., Pate, J. S.: Nitrogen metabolism of nodulated white clover in the presence and absence of nitrate nitrogen. Occasional symposium No. 6 of the British Grassland Society pp. 71–77, British Grassland Society. Hurley, Berkshire (1970)Google Scholar
  3. Dart, P. J., Wildon, D. C.: Nodulation and nitrogen fixation by Vigna sinensis and Vicia atropurpurea: The influence of concentration, form and site of application of combined nitrogen. Austr. J. Agric. Res. 21, 45–56 (1969)Google Scholar
  4. Drozd, J. W., Tubb, R. S., Postgate, J. R.: A chemostat study of the effect of fixed nitrogen sources on nitrogen fixation, membranes and free amino acids in Azotobacter chroococcum. J. gen. Microbiol. 73, 221–232 (1972)Google Scholar
  5. Gibson, A. H., Nutman, P. S.: Studies on the physiology of nodule formation. VII. A reappraisal of the effect of preplanting. Ann. Bot. 24, 420–433 (1960)Google Scholar
  6. Hardy, R. W. F., Burns, R. C., Holsten, R. D.: Application of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol. Biochem. 5, 47–81 (1973)Google Scholar
  7. Hardy, R. W. F., Holsten, R. D., Jackson, E. K., Burns, R. C.: The acetylene-ethylene assay for N2-fixation: Laboratory and field evaluation. Plant Physiol. 43, 1185–1207 (1968)Google Scholar
  8. Jensen, H. L.: Nitrogen fixation in leguminous plants. I. General characters of root nodule bacteria isolated from species of Medicago and Trifolium in Australia. Proc. Linn. Soc. N.S.W. 66, 98–108 (1942)Google Scholar
  9. Keister, D. J.: Acetylene reduction by pure cultures of Rhizobia. J. Bacteriol. 123, 1265–1268 (1975)Google Scholar
  10. Kennedy, I. R.: Primary products of symbiotic nitrogen fixation. II. Pulse-labelling of seradella nodules with 15N2. Biochim. Biophys. Acta. 130, 295–303 (1966)Google Scholar
  11. Kennedy, I. R., Rigaud, J., Trichant, J. C.: Nitrate reductase from bacteriods of Rhizobium japonicum: Enzyme characteristics and possible interactions with nitrogen fixation. Biochim. Biophys. Acta. 397, 24–35 (1975)Google Scholar
  12. Kleiner, D.: Quantitative relations for the repression of nitrogenase synthesis in Azotobacter vinelandii by ammonia. Arch. Microbiol. 101, 153–159 (1974)Google Scholar
  13. Kurz, W. G. W., Rokosh, D. A., LaRue, T. A.: Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids. Can. J. Microbiol. 21, 1009–1012 (1975)Google Scholar
  14. Munns, D. N.: Nodulation of Medicago sativa in solution culture. II. Compensating effects of nitrate and of prior nodulation. Plant Soil. 28, 246–257 (1968)Google Scholar
  15. Pate, J. S., Dart, P. J.: Nodulation studies in legumes. IV. The influence of inoculum strain and time of application of ammonium nitrate on symbiotic response. Plant Soil. 15, 329–346 (1961)Google Scholar
  16. Rigaud, J., Bergersen, F. J., Turner, G. L., Daniel, R. M.: Nitrate dependent anaerobic acetylene-reduction and nitrogen-fixation by soybean bacteriods. J. gen. Microbiol. 77, 137–144 (1973)Google Scholar
  17. Robertson, J. G., Scott, D. B., Farnden, K. J. F.: Ammonia assimilation in lupin nodules. Nature 263, 703–705 (1976)Google Scholar
  18. Robertson, J. G., Warburton, M. P., Farnden, K. J. F.: Induction of glutamate synthase during nodule development in lupin. FEBS Letters 55, 33–37 (1975)Google Scholar
  19. Ryan, E., Fortrell, P. F.: Subcellular localization of enzymes involved in the assimilation of ammonia by soybean root nodules. Phytochemistry 13, 2647–2652 (1974)Google Scholar
  20. Schmidt, G.: On the effect of environmental conditions, especially the nitrogen nutrition, on nodulation and N-linkage of Vicia faba L. Kühn-Arch. 69, 163–222 (1955)Google Scholar
  21. Sorger, G. J.: Regulation of nitrogen fixation in Azotobacter vinelandii OP: The role of nitrate reductase. J. Bacteriol 98, 56–61 (1969)Google Scholar
  22. Van Straten, J., Schmidt, E. L.: Action of water in depressing acetylene reduction by detached nodules. Appl. Microbiol. 29, 432–434 (1975)Google Scholar
  23. Tubb, R. S., Postgate, J. R.: Control of nitrogenase synthesis in Klebsiella pneumoniae. J. gen. Microbiol. 79, 103–117 (1973)Google Scholar
  24. Wilson, P. W.: The biochemistry of symbiotic nitrogen fixation, pp. 114–141. Madison, Wisconsin: The Univ. of Wisconsin Press 1940Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Werner Kamberger
    • 1
  1. 1.Institut für Mikrobiologie der Universität Erlangen-NürnbergErlangenFederal Republic of Germany

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