Archives of Microbiology

, Volume 124, Issue 2–3, pp 161–167

Nitrogen fixation associated with a hotspring green alga

  • Takashi Yamada
  • Kenji Sakaguchi

DOI: 10.1007/BF00427722

Cite this article as:
Yamada, T. & Sakaguchi, K. Arch. Microbiol. (1980) 124: 161. doi:10.1007/BF00427722


A unicellular alga which can grow in the light without a combined nitrogen source was isolated from a hot spring. The cells were almost spherical, usually 5–10 μm in diameter. Absorption spectra of the watersoluble pigments and of the acetone-extracted ones revealed the existence of chlorophyll a and b and the absence of phycobilins. Thin sections examined by electron microscopy revealed an eukaryotic organization with features typical of the coccoid green algae (the Chlorococcales). Cells divided by internal cytokinesis and subsequent liberation of daughter cells from the parental wall, in a way similar to Chlorella. The alga reduced acetylene to ethylene and incorporated 15N2 into cell protoplasm when incubated in a low oxygen atmosphere. Nitrogenase activity was light-dependent, microaerophilic and thermophilic. Although the association of symbiotic nitrogen fixing prokaryotes with the cells may still be possible, any such organisms have not so far been detected.

Key words

Nitrogen fixation Photosynthesis Green alga Chl a and b DCMU Light and O2 dependency 

Abbreviations Used






modified Bristol medium


thin layer chromatography

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Takashi Yamada
    • 1
  • Kenji Sakaguchi
    • 1
  1. 1.Mitsubishi-Kasei Institute of Life SciencesMachida-shi, TokyoJapan

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