Advertisement

Archives of Dermatological Research

, Volume 280, Issue 8, pp 477–480 | Cite as

Studies of polymorphonuclear migration into psoriatic skin using a new in vivo method

  • B. Guillot
  • M. Coupe
  • M. Alirezai
  • J. P. Bureau
  • J. J. Guilhou
Original Contributions

Summary

A new method, employing a skin-implanted cell trap already used to study chemotaxis in cancer patients, was applied to 35 healthy volumteers and 12 psoriatic patients. A dacron disk impregnated with 10 μl of 4–6·106 live BCG suspension was implanted in the deep dermis. After 24 h the disk was removed, and five sections of each disk were counted for polymorphonuclear leukocytes (PMNs) and monocytes. Involved and uninvolved psoriatic skin showed a decrease of PMN migration into the disk as compared with controls. No difference could be demonstrated between involved and uninvolved skin. Mononuclear cell chemotaxis was the same in psoriasis as in controls. These results are in agreement with other in vivo data using mainly the skin chamber technique indicating a decrease of PMN chemotaxis in psoriatic skin at 24 h.

Key words

Polymorphonuclear leukocytes Chemotaxis Psoriasis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Brain SD, Camp RDR, Dowd PM, Black AK, Woullard PM, Mallet AI, Greaves MW (1982) Psoriasis and leukotriene B4. Lancet 2:762–763Google Scholar
  2. 2.
    Breathnach SM, Carrington P, Black MM (1981) Neutrophil leukocyte migration in psoriasis vulgaris. J Invest Dermatol 76:271–274Google Scholar
  3. 3.
    Bureau JP, Senelar R, Cupissol D (1980) Restoration by ketoprofen of defective neutrophil granulocyte migration induced in Guinea pigs by plasma from cancer patient. Br J Exp Pathol 61:479–485Google Scholar
  4. 4.
    Bureau JP, Senelar R, Cupissol D (1981) Plasma transferable inhibition of BCG-induced subcuteanous inflammation in human cancer. J Pathol 133:215–227Google Scholar
  5. 5.
    Bureau JP, Blotman F, Poubelle P, Cupissol D, Senelar R, Simon L (1981) “In vivo” inflammatory cell migration inhibition in rheumatoid arthritis — preliminary results. Rev Rhum 6: A2-III (Abstract) 0063Google Scholar
  6. 6.
    Bureau JP, Coupe M, Garrelly L, Labrecque G (1984) Circannual rhythms of PMN migration induced by BCG in intact mice. Annu Rev Chronopharmacol:337–340Google Scholar
  7. 7.
    Csato M, Dobozy A, Raith L, Simon N (1985) The functions of polymorphonuclear leukocytes emigrating into the skin. Acta Derm Venereol 65:106–110Google Scholar
  8. 8.
    Dubertret L, Lebreton BA, Touraine R (1982) Neutrophil studies in psoriatics: in vivo migration, phagocytosis and bacterial killing. J Invest Dermatol 79:74–78Google Scholar
  9. 9.
    Fraki JE, Jakoi L, Davies AO, Lefkowitz RJ, Snyderman R, Lazarus GS (1983) Polymorphonuclear leukocyte function in psoriasis: chemotaxis chemokinesis, beta-adrenergic receptors, and proteolytic enzymes of polymorphonuclear leukocytes in the peripheral blood from psoriatic patients. J Invest Dermatol 81:254–257Google Scholar
  10. 10.
    Guilhou JJ, Clot J, Meynadier J, Lapinski H (1976) Immunological aspects of psoriasis. I. Immunoglobulins and anti-IgA functions Br J Dermatol 94:501–507Google Scholar
  11. 11.
    Guilhou JJ, Clot J, Guillot B, Andary M, Meynadier J (1980) Immunological aspects of psoriasis. IV. Presence of circulating immune complexes in patients before and after PUVA therapy; correlations with T-cell markers. Br J Dermatol 102:173–178Google Scholar
  12. 12.
    Guillot B, Guilhou JJ, Vendrel JP, Meynadier J (1983) Neutrophil chemotaxis in psoriasis before and after PUVA therapy. Arch Dermatol Res 275:19–22Google Scholar
  13. 13.
    Hanlon SM, Panayi GS, Laurent R (1980) Defective polymorphonuclear leukocyte chemotaxis in rheumatoid arthritis associated with a serum inhibitor. Ann Rheum Dis 39:68–74Google Scholar
  14. 14.
    Kawohl G, Szperalski B, Schroder JM, Christophers E (1980) Polymorphonuclear leukocyte chemotaxis in psoriasis: enhancement by self-activated serum. Br J Dermatol 103:527–533Google Scholar
  15. 15.
    Kemps AS, Cripps AW, Brown S (1980) Suppression of leukocyte chemokinesis and chemotaxis by human IgA. Clin Exp Immunol 40:388–395Google Scholar
  16. 16.
    Krueger GG, Hill HR, Jederberg WW (1978) Inflammatory and immune cell function in psoriasis. A subtle disorder I. In vivo and in vitro survey. J Invest Dermatol 71:189–194Google Scholar
  17. 17.
    Lammers AM, Van de Kerkhof PCM (1987) Response of polymorphonuclear leukocytes to topical leukotriene B4 in healthy and psoriatic skin. Br J Dermatol 116:521–524Google Scholar
  18. 18.
    Langner A, Chorzelski TP, Fraczykowska M, Jablonska S, Szymanczyk J (1983) Is chemotactic activity in polymorphonuclar leukocytes increased in psoriasis? Arch Dermatol Res 275:226–228Google Scholar
  19. 19.
    Lazarus GS, Yost FS, Thomas CA (1977) Polymorphonuclear leukocytes: possible mechanism of accumulation in psoriasis. Science 198:1162–1163Google Scholar
  20. 20.
    Michaelson G (1980) Increased chemotactic activity of neutrophil leukocytes in psoriasis. Br J Dermatol 103:351–356Google Scholar
  21. 21.
    Petrini N, Acampa D, Baldini MA, Fabbri P (1983) Leukocyte chemotaxis in psoriasis vulgaris and in pustular psoriasis. 2nd European Symposium on Psoriasis, Trieste, 7–9 OctoberGoogle Scholar
  22. 22.
    Pigatto PD, Riva F, Altomare GF, Brugo AM, Morandotti A, Finzi AF (1983) Effects of etretinate on chemotaxis of neutrophils from patients with pustular and vulgar psoriasis. J Invest Dermatol 81:418–419Google Scholar
  23. 23.
    Pigatto PD, Radaelli A, Tadini G, Polenghi MM, Brambilla L, Altomare G, Carandente F (1985) Circadian rhythm of the in vivo migration of neutrophils in psoriatic patients. Arch Dermatol Res 277:185–189Google Scholar
  24. 24.
    Preissner WC, Schroder JM, Christophers E (1983) Altered polymorphonuclear leukocyte responses in psoriasis: chemotaxis and degranulation. Br J Dermatol 109:1–8Google Scholar
  25. 25.
    Schroder JM, Szperalski B, Koh CJ, Christophers E (1981) IgA-associated inhibition of polymorphonuclear leukocyte chemotaxis in neutrophilic dermatoses. J Invest Dermatol 77:464–468Google Scholar
  26. 26.
    Senelar R, Bureau JP (1979) In vivo effect of human chorionic gonadotropin on the migration of inflammatory cells in intact or castrated male and female guinea-pigs. A quantitative histological study. I. Study of intact male and female guinea-pigs. Br J Exp Pathol 60:483–488Google Scholar
  27. 27.
    Senelar R, Bureau JP (1979) In vivo effect of human chorionic gonadotropin on the migration of inflammatory cells in intact or castrated male and female guinea-pigs. A quantitative histological study. II. Study of castrated males and females. Br J Exp Pathol 60:489–492Google Scholar
  28. 28.
    Silny W, Pehamberger H, Zielinsky CH, Gschnait F (1980) Effect of PUVA treatment on the locomotion of polymorphonuclear leukocytes and mononuclear cells in psoriasis. J Invest Dermatol 75:187–188Google Scholar
  29. 29.
    Tagami H, Ofuji S (1976) Leukotactic properties of soluble substances in psoriasis scale. Br J Dermatol 95:1–8Google Scholar
  30. 30.
    Tagami H, Kitano Y, Suchisa S, Oku T, Yamada M (1982) Psoriatic leukotactic factor. Further physiochemical characterization and effect on the epidermal cells. Arch Dermatol Res 272:201–213Google Scholar
  31. 31.
    Tigalonowa M, Glinski W, Jablonska S (1983) In vivo mobilization of polymorphonuclear leukocytes in psoriasis: relationship to clinical parameters and serum inhibitory factors. J Invest Dermatol 81:6–9Google Scholar
  32. 32.
    Van Epps DE, Williams RC (1976) Suppression of leukocyte chemotaxis by human IgA myeloma component. J Exp Med 144:1227–1242Google Scholar
  33. 33.
    Wahba A, Cohen HA, Bar-Eli M, Gallily R (1978) Enhanced chemotactic and phagocytic activities of leukocytes in psoriasis vulgaris. J Invest Dermatol 71:186–188Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • B. Guillot
    • 1
  • M. Coupe
    • 1
  • M. Alirezai
    • 1
  • J. P. Bureau
    • 1
  • J. J. Guilhou
    • 1
  1. 1.Service de Dermatologie-PhlébologieHôpital Saint-CharlesMontpellier CedexFrance

Personalised recommendations