Acta Neuropathologica

, Volume 84, Issue 1, pp 39–44 | Cite as

Latent herpes simplex virus type 1 in human geniculate ganglia

  • Y. Furuta
  • T. Takasu
  • K. C. Sato
  • S. Fukuda
  • Y. Inuyama
  • K. Nagashima
Regular Papers

Summary

Viral infection, especially by reactivation of herpes simplex virus (HSV) has been considered to be a possible explanation for the pathogenesis of idiopathic peripheral facial nerve palsy (Bell's palsy). We investigated whether the geniculate ganglia of man contain latent HSV type 1 (HSV-1), and compared the frequency of HSV-infected ganglia and that of latently infected neurons in human geniculate ganglia and in trigeminal ganglia. From autopsy specimens of eight adults 15 geniculate ganglia and 16 trigeminal ganglia were examined by means of in situ hybridization and immunohistochemical staining. The HSV-1 genome was detected in 11 of the 15 (71%) geniculate ganglia and in 13 of the 16 (81%) trigeminal ganglia. No HSV antigen was noted in any of the ganglia. The incidence of latently infected neurons was 0.9% in the trigeminal ganglia and 5.3% in the geniculate ganglia. The difference in percentages between the two types of ganglia was significant. Our results suggest that reactivation of latent HSV in the geniculate ganglia is a probable cause of some cases of herpetic stomatitis and of idiopathic peripheral facial nerve palsy.

Key words

Herpes simplex virus Latent infection Human geniculate ganglion In situ hybridization Bell's palsy 

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References

  1. 1.
    Adour KK, Bell DN, Hilsinger RL (1975) Herpes simplex virus in idiopathic facial paralysis (Bell palsy). JAMA 233:527–530Google Scholar
  2. 2.
    Baringer JR, Swoveland P (1973) Recovery of herpes-simplex virus from human trigeminal ganglions. N Engl J Med 288:648–650Google Scholar
  3. 3.
    Bastian FO, Rabson AS, Yee CL, Tralka TS (1972) Herpesvirus hominis: isolation from human trigeminal ganglion. Science 178:306–307Google Scholar
  4. 4.
    Cook ML, Bastone VB, Stevens JG (1974) Evidence that neurons harbor latent herpes simplex virus. Infect Immun 9:946–951Google Scholar
  5. 5.
    Cox KH, DeLeon DV, Angerer IM (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502Google Scholar
  6. 6.
    Croen KD, Ostrove JM, Dragovic LJ, Smialek JE, Straus SE (1987) Latent herpes simplex virus in human trigeminal ganglia. Detection of an immediate early gene “anti-sense” transcript by in situ hybridization. N Engl J Med 317:1427–1432Google Scholar
  7. 7.
    Deatly AM, Spivack JG, Lavi E, O'Boyle DRII, Fraser NW (1988) Latent herpes simplex virus type 1 transcripts in peripheral and central nervous system tissues of mice map to similar regions of the viral genome. J Virol 62:749–756Google Scholar
  8. 8.
    Degré M, Bukholm G, Lund EE, Djupesland G (1978) Attempts to isolate virus from human trigeminal and facial ganglions. Med Microbiol Immunol 166:151–156Google Scholar
  9. 9.
    Djupesland G, Berdal P, Johannessen TA, Degré M, Stien R, Skrede S (1976) Viral infection as a cause of acute peripheral facial palsy. Arch Otolaryngol 102:403–406Google Scholar
  10. 10.
    Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13Google Scholar
  11. 11.
    Furuta Y, Shinohara T, Sano K, Meguro M, Nagashima K (1990) In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 43:806–809Google Scholar
  12. 12.
    Galloway DA, Fenoglio C, Shevchuk M, McDougall JK (1979) Detection of herpes simplex RNA in human sensory ganglia. Virology 95:265–268Google Scholar
  13. 13.
    McCormick DP (1972) Herpes-simplex virus as cause of Bell's palsy. Lancet I:937–939Google Scholar
  14. 14.
    Palva T, Hortling L, Ylikoski J, Colan Y (1978) Viral culture and electron microscopy of ganglion cells in Menier's disease and Bell's palsy. Acta Otolaryngol 86:269–275Google Scholar
  15. 15.
    Post LE, Conley AJ, Mocarski ES, Roizman B (1980) Cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as BamHI fragments. Proc Natl Acad Sci USA 77:4201–4205Google Scholar
  16. 16.
    Rentrop M, Knapp B, Winter H, Schweizer J (1986) Aminoalkysilane-treated glass slides as support for in situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions. Histochem J 18:271–276Google Scholar
  17. 17.
    Rock DL, Nesburn AB, Ghiasi H, Ong J, Lewis TL, Lokensgard JR, Weschsler SL (1987) Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1. J Virol 61:3820–3826Google Scholar
  18. 18.
    Sequiera LW, Jennings LC, Carrasco LH, Lord MA, Curry A, Sutton RNP (1979) Detection of herpes-simplex viral genome in brain tissue. Lancet II:609–612Google Scholar
  19. 19.
    Spivack JG, Fraser NW (1987) Detection of herpes simplex virus type 1 transcripts during latent infection in mice. J Virol 61:3841–3847Google Scholar
  20. 20.
    Stevens JG, Wagner EK, Devi-Rao GB, Cook ML, Feldman LT (1987) RNA complementary to a herpesvirus α gene mRNA is prominent in latently infected neurons. Science 235:1056–1059Google Scholar
  21. 21.
    Stevens JG, Haarr L, Porter DD, Cook ML, Wagner EK (1988) Prominence of the herpes simplex virus latency-associated transcript in trigeminal ganglia from seropositive humans. J Infect Dis 158:117–123Google Scholar
  22. 22.
    Vahlne A, Edström S, Arstila P, Beran M, Ejnell H, Nylen O, Lycke E (1981) Bell's palsy and herpes simplex virus. Arch Otolaryngol 107:79–81Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Y. Furuta
    • 1
    • 2
  • T. Takasu
    • 1
    • 2
  • K. C. Sato
    • 1
  • S. Fukuda
    • 2
  • Y. Inuyama
    • 2
  • K. Nagashima
    • 1
  1. 1.Department of PathologyHokkaido University School of MedicineSapporoJapan
  2. 2.Department of OtolaryngologyHokkaido University School of MedicineSapporoJapan

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