Molecular and General Genetics MGG

, Volume 103, Issue 2, pp 105–115 | Cite as

Genetic analysis of lon mutants of strain K-12 of Escherichia coli

  • John Donch
  • Joseph Greenberg
Article
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Revised:

Summary

Following UV irradiation of AB1157 31 mucoid ultraviolet light UV sensitive mutants were isolated. These were all induced to form filaments by UV irradiation, i.e. they had all the phenotypic properties of Lon mutants. These lon mutants fell into two phenotypic classes based on their sensitivity to UV. The gene determining UV sensitivity and mucoidy in all mutants of both Class A and Class B was cotransducible with proC. Intra-class crosses by Pl transduction yielded no UV resistant recombinants. Inter-class crosses yielded UV resistant nonmucoid recombinants, the frequency depending on the direction of the cross. The data imply two adjacent blocks in the lon region of E. coli and the order of markers in this region is probably proC tsx lon Class A lon purE Class B.

Keywords

Genetic Analysis Phenotypic Property Phenotypic Classis Adjacent Block Sensitive Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • John Donch
    • 1
  • Joseph Greenberg
    • 1
  1. 1.Palo Alto Medical Research FoundationPalo Alto

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