Molecular and General Genetics MGG

, Volume 215, Issue 3, pp 517–528 | Cite as

Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria

  • Edna Ben Asher
  • Olga Groudinsky
  • Geneviève Dujardin
  • Nicola Altamura
  • Michèle Kermorgant
  • Piotr P. Slonimski
Article

Summary

We have cloned three distinct nuclear genes, NAM1, NAM7, and NAM8, which alleviate mitochondrial intron mutations of the cytochrome b and COXI (subunit I of cytochrome oxidase) genes when present on multicopy plasmids. These nuclear genes show no sequence homology to each other and are localized on different chromosomes: NAM1 on chromosome IV, NAM7 on chromosome XIII and NAM8 on chromosome VIII. Sequence analysis of the NAM1 gene shows that it encodes a protein of 440 amino acids with a typical presequence that would target the protein to the mitochondrial matrix. Inactivation of the NAM1 gene by gene transplacement leads to a dramatic reduction of the overall synthesis of mitochondrial protein, and a complete absence of the COXI protein which is the result of a specific block in COXI pre-mRNA splicing. The possible mechanisms by which the NAM1 gene product may function are discussed.

Key words

Yeast Nuclear genes Mitochondrial translation Mitochondrial splicing Suppression 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Edna Ben Asher
    • 1
  • Olga Groudinsky
    • 1
  • Geneviève Dujardin
    • 1
  • Nicola Altamura
    • 1
  • Michèle Kermorgant
    • 1
  • Piotr P. Slonimski
    • 1
  1. 1.Centre de Génétique Moléculaire du C.N.R.S.Laboratoire propre associé à l'Université P. et M. CurieGif-sur-Yvette CedexFrance
  2. 2.Department of GeneticsThe Weizmann InstituteRehovotIsraél
  3. 3.Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, c/o Dipartimento di Biochimica e Biologia MolecolareUniversità di BariBariItaly

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