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Archives of Dermatological Research

, Volume 280, Issue 6, pp 358–362 | Cite as

Purification of rat cutaneous mast cells with Percoll density centrifugation

  • H. Hachisuka
  • M. Kusuhara
  • M. Higuchi
  • K. Okubo
  • Y. Sasai
Original Contributions

Summary

The skin is the major site on anaphylaxis, and cutaneous mast cells have an important role in its reactions. The isolation and purification of rat cutaneous mast cells are described here. Rat abdominal skin was digested with collagenase and hyaluronidase, and centrifuged with Percoll. The buoyant density of cutaneous mast cells was high, and relatively pure mast cells were obtained. The purity of cutaneous mast cells was 7.4%±2.4% before and 50.0%±6.4% after Percoll density centrifugation; peritoneal mast cells revealed 5.8%±1.3% purity before and 61.0%±10.6% purity after the same procedure. The isolated cutaneous cells released 21.3%±3.8% histamine and the peritoneal mast cells released 55.5%±3.8% histamine upon stimulation with 10 μg/ml compound 48/80. These findings suggest that there are functional subsets of connective tissue mast cells.

Key words

Mast cell isolation Skin Percoll 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • H. Hachisuka
    • 1
  • M. Kusuhara
    • 1
  • M. Higuchi
    • 1
  • K. Okubo
    • 1
  • Y. Sasai
    • 1
  1. 1.Cutaneous Biology Unit, Department of DermatologyKurume University School of MedicineKurumeJapan

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