Biochemical and biological profile of a new enzyme preparation from antarctic krill (E. superba) suitable for debridement of ulcerative lesions
- 123 Downloads
A protease extract from Antarctic krill (E. superba) intended as a new enzymatic debrider for necrotic ulcers has been characterized by sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis and fast protein liquid chromatography. The predominant enzymes in the preparation represent trypsin-like activity associated with three serine proteinases. In addition two carboxypeptidases A and B are present as cooperative enzymes for a more complete breakdown of complex proteinaceous substrates. Biological studies on a well-defined substrate (fibrin) originating from leg ulcers, demonstrated more effective degradation by krill enzymes than bovine trypsin, a common component in marketed enzymatic debriders. These findings support previously in vitro/in vivo studies in an animal model (rat) using excised rat skin as “necrotic” tissue.
Key wordsAntarctic krill (E. superba) Proteases Biochemical/biological profile Enzymatic debridement
p-toluene-sulfonyl-l-arginine methyl ester; hipp-l-phe hippuryl-l-phenylalanine; hipp-l-arg, hippuryl-l-arginine
sodium dodecyl sulphate
Unable to display preview. Download preview PDF.