When A. pullulans is grown on a glucose medium with a limiting nitrogen source and low buffer capacity, the yeast-like cells that are originally present undergo a transition to chlamydospores. The initial pH must be around 6 for the transition to take place under optimal conditions. On the above-mentioned medium pH decreases to values below 2 in the first two days; if this decrease is prevented, either by buffering the medium or by repeatedly adjusting the pH to its original value no chlamydospores form.
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Bermejo, J. M., Dominguez, J. B., Goñi, F. M. and Uruburu, F. 1981. Influence of carbon and nitrogen sources on the transition from yeast-like cells to chlamydospores in Aureobasidium pullulans. — Antonie van Leeuwenhoek 47: 107–119.
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Bermejo, J.M., Dominguez, J.B., Goñi, F.M. et al. Influence of pH on the transition from yeast-like cells to chlamydospores in Aureobasidium pullulans . Antonie van Leeuwenhoek 47, 385–392 (1981). https://doi.org/10.1007/BF00426000
- Optimal Condition
- Nitrogen Source
- Buffer Capacity