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Molecular and General Genetics MGG

, Volume 180, Issue 2, pp 449–453 | Cite as

Molybdenum hydroxylases in Drosophila

I. Preliminary studies of pyridoxal oxidase
  • Cynthia K. Warner
  • Deborah T. Watts
  • Victoria Finnerty
Article

Summary

A reliable method for visualizing the Drosophila enzyme pyridoxal oxidase in polyacrylamide gels is described. Antiserum to pyridoxal oxidase has been produced and used in quantitative immunoelectrophoresis to determine the relative amounts of pyridoxal oxidase cross reacting material (CRM) in several mutants including lpo, lxd, ma-l and cin. The lpo variant did not have CRM for PO, thus further supporting the idea that it represents a structural gene for pyridoxal oxidase in Drosophila. CRM for PO was found in ma-l and lxd indicating that their effects upon the enzyme are probably post-translational. No CRM for PO could be found in the cin mutants.

Keywords

Enzyme Molybdenum Polyacrylamide Structural Gene Reliable Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Cynthia K. Warner
    • 1
  • Deborah T. Watts
    • 1
  • Victoria Finnerty
    • 1
  1. 1.Department of BiologyEmory UniversityAtlantaUSA

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