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Molecular and General Genetics MGG

, Volume 180, Issue 2, pp 391–398 | Cite as

Physical mapping of the BglI, BglII, PstI and EcoRI restriction fragments of staphylococcal phage ϕ11 DNA

  • Brigitte Bächi
Article

Summary

A cleavage map of the generalized transducing staphylococcal phage ϕ11 DNA has been constructed by reciprocal double digestion. All three BglI, the six BglII, the three PstI, and 11 out of 15 EcoRI sites have been mapped. The map is circular, with a total length of 42 kb, and has been divided into 100 map units. The phage DNA is cyclically permuted and has a terminal redundancy of about 11 kb. The preferential starting point and direction for packaging DNA lies at map unit 79 and proceeds towards higher map units.

Keywords

Physical Mapping Restriction Fragment EcoRI Site EcoRI Restriction Generalize Transduce 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Brigitte Bächi
    • 1
  1. 1.Institute of Medical MicrobiologyUniversity of ZurichZurichSwitzerland

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