Molecular and General Genetics MGG

, Volume 247, Issue 1, pp 114–117 | Cite as

Structural analysis of Tpn1, a transposable element isolated from Japanese morning glory bearing variegated flowers

  • Atsushi Hoshino
  • Yoshishige Inagaki
  • Shigeru Iida
Short Communication

Abstract

The 6.4 kb transposable element Tpn1 belonging to the En/Spm family was found within one of the DFR (dihydroflavonol-4-reductase) genes for anthocyanin biosynthesis in a line of Japanese morning glory (Pharbitis nil) bearing variegated flowers. Sequencing of the Tpn1 element revealed that it is 6412 by long and carries 28-bp perfect terminal inverted repeats. Its subterminal repetitive regions, believed to be the cis-acting sequences for transposition, show striking structural features. Twenty-two copies of the 10-bp sequence motif GACAACGGTT can be found as direct or inverted repeats within 650 by of the 5′ end of the element, and 33 copies of the sequence motif lie within 800 by of the 3′ terminus. All these 22 copies of the sequence motif near the 5′ terminus and 30 copies in the 3′ terminal region are arranged as inverted repeats and 3–8 by AT-rich sequences are detected between these inverted repeats. In addition, four copies of 122-bp tandem repeats and six copies of 104-bp tandem repeats are present in the 5′ and 3′ subterminal repetitive regions, respectively. No large open reading frame characteristic of autonomous elements of the En/Spm family can be detected within the element. The results are discussed with respect to heritable changes in flower variegation in this line of Japanese morning glory.

Key words

Transposable element Tpn1 structure Japanese morning glory En/Spm family Subterminal repetitive region 

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References

  1. Coen ES, Robbins TP, Almeida J, Hudson A, Carpenter R (1989) Consequences and mechanisms of transposition in Antirrhinum majus. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington, DC, pp 413–436Google Scholar
  2. Fedoroff NV (1989) Maize transposable elements. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington, DC, pp 375–411Google Scholar
  3. Frey M, Reinecke J, Grant S, Saedler H, Gierl A (1990) Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins. EMBO J 9:4037–4044Google Scholar
  4. Gierl A, Saedler H (1992) Plant-transposable elements and gene tagging. Plant Mol Biol 19:39–49Google Scholar
  5. Gierl A, Lütticke S, Saedler H (1988) TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein. EMBO J 7:4045–4053Google Scholar
  6. Hagiwara T (1956) Genes and chromosome maps in the Japanese morning glory. Bull Res Coll Agric Vet Sci Nihon Univ 5:34–56Google Scholar
  7. Imai Y (1934) On the mutable genes of Pharbitis, with special reference to their bearing on the mechanism of bud-variation. J Coll Agric Imp Univ Tokyo 12:479–523Google Scholar
  8. Inagaki Y, Hisatomi Y, Suzuki T, Kasahara K, Iida S (1994) Isolation of a Suppressor-mutator/Enhancer-like transposable element, Tpn1, from Japanese morning glory bearing variegated flowers. Plant Cell 6:375–383Google Scholar
  9. Krebbers E, Hehl R, Piotrowiak R, Lönnig WE, Sommer H, Saedler H (1987) Molecular analysis of parammant plants of Antirrhinum majus and the involvement of transposable elements. Mol Gen Genet 209:499–507Google Scholar
  10. Luo D, Coen ES, Doyle S, Carpenter R (1991) Pigmentation mutants produced by transposon mutagenesis in Antirrhinum majus. Plant J 1:59–69Google Scholar
  11. Masson P, Surosky R, Kingsbury JA, Fedoroff NV (1987) Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus. Genetics 117:117–137Google Scholar
  12. Masson P, Rutherford G, Banks JA, Fedoroff N (1989) Essential large transcripts of the maize Spm transposable element are generated by alternative splicing. Cell 58:755–765Google Scholar
  13. Masson P, Strem M, Fedoroff N (1991) The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco. Plant Cell 3:73–85Google Scholar
  14. Nacken WKF, Piotrowiak R, Saedler H, Sommer H (1991) The transposable element Tam1 from Antirrhinum majus shows structural homology to the maize transposon En/Spm and has no sequence specificity of insertion. Mol Gen Genet 228:201–208Google Scholar
  15. Nevers P, Shepherd NS, Saedler H (1986) Plant transposable elements. Adv Bot Res 12:103–203Google Scholar
  16. Pereira A, Schwarz-Sommer Z, Gierl A, Bertram I, Peterson PA, Saedler H (1985) Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays. EMBO J 4:17–23Google Scholar
  17. Pereira A, Cuypers H, Gierl A, Schwarz-Sommer Z, Saedler H (1986) Molecular analysis of the En/Spm transposable element system of Zea mays. EMBO J 5:835–841Google Scholar
  18. Peterson P (1987) Mobile elements in plants. CRC Crit Rev Plant Sci 6:104–208Google Scholar
  19. Rhodes PR, Vodkin LO (1985) Highly structured sequence homology between an insertion element and the gene in which it resides. Proc Natl Acad Sci USA 82:493–497Google Scholar
  20. Rhodes PR, Vodkin LO (1988) Organization of the Tgm family of transposable elements in soybean. Genetics 120:597–604Google Scholar
  21. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  22. Schäppi M, Raina R, Fedoroff N (1994) Epigenetic regulation of the maize Spm transposable element: novel activation of a methylated promoter by TnpA. Cell 77:427–437Google Scholar
  23. Shirsat AH (1988) A transposon-like structure in the 5′ flanking sequence of a legumin gene from Pisum sativum. Mol Gen Genet 212:129–133Google Scholar
  24. Trentmann SM, Saedler H, Gierl A (1993) The transposable element En/Spm-encoded TNPA protein contains a DNA binding and a dimerization domain. Mol Gen Genet 238:201–208Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Atsushi Hoshino
    • 1
  • Yoshishige Inagaki
    • 1
    • 2
  • Shigeru Iida
    • 1
  1. 1.Department of Biological Science and TechnologyScience University of TokyoYamazaki, Noda-shi, ChibaJapan
  2. 2.Department of Health Science, Faculty of Pharmaceutical SciencesUniversity of TokyoHongo, Bunkyo-ku, TokyoJapan

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