Advertisement

Archives of Microbiology

, Volume 137, Issue 1, pp 79–84 | Cite as

A dissimilatory nitrite reductase in Paracoccus halodenitrificans

  • Michael A. Grant
  • L. I. Hochstein
Original Papers

Abstract

Paracoccus halodenitrificans produced a membrane-associated nitrite reductase. Spectrophotometric analysis showed it to be associated with a cd-cytochrome and located on the inner side of the cytoplasmic membrane. When supplied with nitrite, membrane preparations produced nitrous oxide and nitric oxide in different ratios depending on the electron donor employed. The nitrite reductase was maximally active at relatively low concentrations of sodium chloride and remained attached to the membranes at 100 mM sodium chloride.

Key words

Denitrification Halophile Nitrite reductase Paracoccus halodenitrificans cd-Cytochrome 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cresswell CF, Hageman RH, Hewitt EJ, Hucklesby DP (1965) The reduction of nitrate, nitrite, and hydroxylamine to ammonia by enzymes from Cucurbita pepo L. in the presence of reduced benzyl viologen as electron donor. Biochem J 94:40–53Google Scholar
  2. Firestone MK (1982) Biological denitrification. In: Stevenson FJ (ed) Nitrogen in agricultural soils. American Society for Agronomy, Madison, Wisconsin, pp 289–326Google Scholar
  3. Iwasaki H, Matsubara T (1971) Cytochrome c-557 (551) and cytochrome cd of Alcaligenes feacalis. J Biochem (Tokyo) 69:847–857Google Scholar
  4. Iwasaki H, Matsubara T (1972) A nitrite reductase from Achromobacter cycloclastes. J Biochem 71:645–652Google Scholar
  5. Knowles R (1982) Denitrification. Microbiol Rev 46:43–70Google Scholar
  6. Kodama T (1970) Effects of growth conditions on formation of cytochrome system of a denitrifying bacterium, Pseudomonas stutzeri. Plant Cell Physiol 11:231–239Google Scholar
  7. Koike I, Hattori A (1975) Energy yield of denitrification: an estimate from growth yields in continuous culture of Pseudomonas denitrificans under nitrate, nitrite, and nitrous oxide-limited conditions. J Gen Microbiol 88:11–19Google Scholar
  8. Lam Y, Nicholas DJD (1969) A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans. Biochim Biophys Acta 180:459–472Google Scholar
  9. Le Gall J, Payne WJ, Morgan v, Dervartanian D (1979) On the purification of nitrite reductase from Thiobacillus denitrificans and its reaction with nitrite under reducing conditions. Biochem Biophys Res Comm 87:335–362Google Scholar
  10. Lowry O, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  11. Payne WJ (1981) Denitrification. Wiley-Interscience, New York, NYGoogle Scholar
  12. Sadler M, McAninch M, Alico R, Hochstein LI (1980) The intracellular Na+ and K+ composition of the moderately halophilic bacterium Paracoccus halodenitrificans. Can J Microbiol 26:496–502Google Scholar
  13. Sawhney V, Nicholas DJD (1978) Sulphide-linked nitrite reductase from Thiobacillus denitrificans with cytochrome oxidase activity: purification and properties. J Gen Microbiol 106:119–128Google Scholar
  14. Showe MK, DeMoss JA (1968) Localization and regulation of synthesis of nitrate reductase in Escherichia coli. J Bacteriol 95:1305–1313Google Scholar
  15. Wharton DC, Weintraub ST (1980) Identification of nitric oxide and nitrous oxide as products of nitrite reduction by Pseudomonas cytochrome oxidase (nitrite reductase). Biochem Biophys Res Comm 97:236–242Google Scholar
  16. Yamanaka T, Okunuki K (1963) Crystalline Pseudomonas cytochrome oxidase. I. Enzymatic properties with special reference to the biological specificity. Biochim Biophys Acta 67:366–378Google Scholar
  17. Zumft WG, Sherr BF, Payne WJ (1979) A reappraisal of the nitricoxide binding protein of denitrifying Pseudomonas. Biochem Biophys Res Comm 88:1230–1236Google Scholar
  18. Zumft WG, Frunzke K (1982) Discrimination of ascorbateduction of nitric oxide in denitrifying Pseudomonas perfectomarinus. Biochim Biophys Acta 681:459–468Google Scholar
  19. Zumft WG, Vega JM (1979) Reduction of nitrite to nitrous oxide by a cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus. Biochim Biophys Acta 548: 484–499Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Michael A. Grant
    • 1
  • L. I. Hochstein
    • 1
  1. 1.Extraterrestrial Research DivisionNASA Ames Research CenterMoffett FieldUSA

Personalised recommendations