Molecular and General Genetics MGG

, Volume 191, Issue 3, pp 519–524

Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe

  • Eiko Otaka
  • Ken-ichi Higo
  • Takuzi Itoh
Article

Summary

The cytoplasmic ribosomal proteins from a fission yeast Schizosaccharomyces pombe were analysed by two-dimensional polyacrylamide gel electrophoresis. Seventy-three protein species were identified in the 80S ribosome, and named SP-S1 to SP-S33 and SP-L1 to SP-L40 in the small and large subunits, respectively. Many of these proteins could be correlated to those of Saccharomyces cerevisiae on the basis of their electrophoretic mobilities. Eleven proteins were isolated from the 80S ribosome, and their amino acid compositions were determined. Of these, SP-S6, SP-L1, SP-L12, SP-L15, SP-L17, SP-L27, SP-L36 and SP-L40c and d were sequenced from their amino-termini. SP-S28 and SP-L2 appear to have their amino-termini blocked. These results were compared with the data available for the S. cerevisiae and rat liver ribosomal proteins. The S. cerevisiae counterparts of the eight proteins mentioned above were found to be YS4, YL1, YL10, YL14, YL35, YL40 and YL44c and d, respectively. The rat liver counter-parts of SP-S6, SP-L1, SP-L27 and SP-L40c and d were the rat S6, L4, L37 and P2, respectively. Comparison of the partial sequences of these ribosomal proteins suggests that these two yeasts are relatively far apart, phylogenetically.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barnitz JT, Cramer JH, Rownd RH, Cooley L, Söll D (1982) Arrangement of the ribosomal RNA genes in Schizosaccharomyces pombe. FEBS Lett 143:129–132Google Scholar
  2. Coddington A, Fluri R (1977) Characterization of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis. Mol Gen Genet 158:93–100Google Scholar
  3. Erdmann VA, Huysmans E, Vandenberghe A, De Wachter R (1983) Collection of published 5S and 5.8S ribosomal RNA sequences. Nucl Acids Res 11:r105-r133Google Scholar
  4. Gressner AM, Wool IG (1974) The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycin. Biochem Biophys Res Commun 60:1482–1490Google Scholar
  5. Higo K, Otaka E (1979) Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast. Biochemistry 18:4191–4196Google Scholar
  6. Higo K, Itoh T, Osawa S (1978) Evolution of ribosomal proteins. In: Matsubara H, Yamanaka T (eds) Evolution of protein molecules. Japan Scientific Societies Press, Tokyo, pp 197–207Google Scholar
  7. Higo K, Itoh T, Kumazaki T, Osawa S (1980) Ribosomal proteins from mesophilic and thermophilic bacteria. In: Osawa S, Ozeki H, Uchida H, Yura T (eds) Genetics and evolution of RNA polymerase, tRNA and ribosomes. University of Tokyo Press, Tokyo, pp 655–666Google Scholar
  8. Higo K, Otaka E, Osawa S (1982) Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: Correlation to Eschericha coli 30S proteins. Mol Gen Genet 185:239–244Google Scholar
  9. Hori H, Itoh T, Osawa S (1982) The phylogenic structure of the metabacteria. Zentralbl Bakteriol Parasitenk Infektionskr Hyg Abt I Orig Reihe C3:18–30Google Scholar
  10. Itoh T (1980) Primary structure of yeast acidic ribosomal protein YP A1. FEBS Lett 114:119–123Google Scholar
  11. Itoh T, Higo K, Otaka E (1979) Isolation and characterization of twenty-three ribosomal proteins from large subunits of yeast. Biochemistry 18:5787–5793Google Scholar
  12. Itoh T, Higo K, Otaka E, Osawa S (1980) Studies on the primary structures of yeast ribosomal proteins. In: Osawa S, Ozeki H, Uchida H, Yura T (eds) Genetics and evolution of RNA polymerase, tRNA and ribosomes. University of Tokyo Press, Tokyo, pp 609–624Google Scholar
  13. Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for finger-printing of ribosomal proteins. Anal Biochem 36:401–412Google Scholar
  14. Komiya H, Miyazaki M, Takemura S (1981) The nucleotide sequence of 5S ribosomal RNA from Schizosaccharomyces pombe. J Biochem (Tokyo) 89:1663–1666Google Scholar
  15. Kruiswijk T, Planta RJ (1975) Further analysis of the protein composition of yeast ribosomes. FEBS Lett 58:102–105Google Scholar
  16. Lin A, Wittmann-Liebold B, McNally J, Wool IG (1982) The primary structure of the acidic phosphoprotein P2 from rat liver 60S ribosomal subunits. J Biol Chem 257:9189–9197Google Scholar
  17. Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D (1982) The 5S RNA genes of Schizosaccharomyces pombe. Nucl Acids Res 10:487–500Google Scholar
  18. McConkey EH, Bielka H, Gordon J, Lastick SM, Lin A, Ogata K, Reboud J-P, Traugh JA, Traut RR, Warner JR, Welfle H, Wool IG (1979) Proposed uniform nomenclature for mammalian ribosomal proteins. Mol Gen Genet 169:1–6Google Scholar
  19. Mets LJ, Bogorad L (1974) Two-dimensional polyacrylamide gel electrophoresis: An improved method for ribosomal proteins. Anal Biochem 57:200–210Google Scholar
  20. Ogata K, Terao K (1981) Analytical methods for ribosomal proteins of rat liver 40S and 60S subunits by “three-dimensional” acrylamide gel electrophoresis. In: Moldave K (ed) RNA and protein synthesis. Academic Press, New York, pp 598–610Google Scholar
  21. Otaka E, Kobata K (1978) Yeast ribosomal proteins: I. Characterization of cytoplasmic ribosomal proteins by two-dimensional gel electrophoresis. Mol Gen Genet 162:259–268Google Scholar
  22. Otaka E, Osawa S (1981) Yeast ribosomal proteins: V. Correlation of several nomenclatures and proposal of a standard nomenclature. Mol Gen Genet 181:176–182Google Scholar
  23. Otaka E, Higo K, Osawa S (1982) Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast. Biochemistry 21:4545–4550Google Scholar
  24. Planta RJ, Meyerink JH (1980) Organization of the ribosomal RNA genes in eukaryotes. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes: Structure, function, and Genetics. University Park Press, Baltimore Maryland, pp 871–887Google Scholar
  25. Schaak J, Mao J, Söll D (1982) The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe. Nucl Acids Res 10:2851–2864Google Scholar
  26. Tabata S (1981) Nucleotide sequences of the 5S ribosomal RNA genes and their adjacent regions in Schizosaccharomyces pombe. Nucl Acids Res 9:6429–6437Google Scholar
  27. Warner JR, Gorenstein C (1978) The ribosomal proteins of Saccharomyces cerevisiae. In: Prescott DM (ed) Methods in cell biology, vol 20. Academic Press, New York, pp 45–60Google Scholar
  28. Wittmann-Liebold B, Geissler AW, Lin A, Wool IG (1979) Sequence of the amino-terminal region of rat liver ribosomal proteins S4, S6, S8, L6, L7a, L18, L27, L30, L37, L37a, and L39. J Supramol Struct 12:425–433Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Eiko Otaka
    • 1
  • Ken-ichi Higo
    • 1
  • Takuzi Itoh
    • 1
  1. 1.Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and BiologyHiroshima UniversityHiroshimaJapan

Personalised recommendations