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Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 360–362 | Cite as

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli

  • Yasunobu Kano
  • Setsuo Yoshino
  • Morimasa Wada
  • Kazushige Yokoyama
  • Masahiro Nobuhara
  • Fumio Imamoto
Short Communication

Summary

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.

Keywords

Escherichia Coli Nucleotide Codon Amino Acid Sequence Binding Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Yasunobu Kano
    • 1
  • Setsuo Yoshino
    • 2
  • Morimasa Wada
    • 1
  • Kazushige Yokoyama
    • 1
  • Masahiro Nobuhara
    • 3
  • Fumio Imamoto
    • 1
  1. 1.Laboratory of Molecular Genetics(Riken) The Institute of Physical and Chemical ResearchWako, Saitama
  2. 2.Central Research LaboratoriesMitsui Toatsu Chemicals, Inc.Yokohama, Kanagawa
  3. 3.Research Laboratories of Cell ScienceMochida Pharmaceutical Co., LTDTokyoJapan

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