Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 360–362 | Cite as

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli

  • Yasunobu Kano
  • Setsuo Yoshino
  • Morimasa Wada
  • Kazushige Yokoyama
  • Masahiro Nobuhara
  • Fumio Imamoto
Short Communication


The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.


Escherichia Coli Nucleotide Codon Amino Acid Sequence Binding Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aiba H, Fujimoto S, Ozaki N (1982) Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res 10:1345–1361Google Scholar
  2. Champoux JI (1978) Proteins that affect DNA conformation. Ann Rev Biochem 47:449–479Google Scholar
  3. Griffith JD (1976) Visualization of prokaryotic DNA in a regularly condensed chromatin-like fiber. Proc Natl Acad Sci USA 73:563–567Google Scholar
  4. Hawley DK, McClure WR (1983) Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res 11:2237–2255Google Scholar
  5. Hübscher U, Lutz H, Kornberg A (1980) Novel histone H2A-like protein of Escherichia coli. Proc Natl Acad Sci USA 77:5097–5101Google Scholar
  6. Ikemura T, Ozeki H (1983) Codon usage and transfer RNA contents: Organism-specific codon-choice patterns in reference to the isoacceptor contents. Cold Spring Harbor Symp Quant Biol 47:1087–1097Google Scholar
  7. Kishi F, Ebina Y, Miki T, Nakazawa T, Nakazawa A (1982) Purification and characterization of a protein from Escherichia coli which forms complexes with superhelical and single-stranded DNA. J Biochem 92:1059–1068Google Scholar
  8. Kornberg T, Lockwood A, Worcel A (1974) Replication of the Escherichia coli chromosome with a soluble enzyme system. Proc Natl Acad Sci USA 71:3189–3193Google Scholar
  9. Laine B, Kmiecik D, Sautiere P, Biserte G, Cohen-Solal M (1980) Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli. Eur J Biochem 103:447–461Google Scholar
  10. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York, USAGoogle Scholar
  11. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560Google Scholar
  12. Meagher RB, Tait RC, Betlach M, Boyer HW (1977) Protein expression in E. coli minicells by recombinant plasmids. Cell 10:521–536Google Scholar
  13. Mende L, Timm B, Subramanian AR (1978) Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli. FEBS Lett 96:395–398Google Scholar
  14. Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–77Google Scholar
  15. Pettijohn DE (1982) Structure and properties of the bacterial nucleoid. Cell 30:667–669Google Scholar
  16. Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72:784–788Google Scholar
  17. Rouvière-Yaniv J (1977) Localization of HU protein on the Escherichia coli nucleoid. Cold Spring Harbor Symp Quant Biol 42:439–447Google Scholar
  18. Rouvière-Yaniv J, Gros F (1975) Characterization of a novel, low molecular weight DNA binding protein from Escherichia coli. Proc Natl Acad Sci USA 72:3428–3432Google Scholar
  19. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  20. Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346Google Scholar
  21. Stonington OG, Pettijohn DE (1971) The folded genome of Escherichia coli isolated in a protein-DNA complex. Proc Natl Acad Sci USA 68:6–9Google Scholar
  22. Takanami M, Sugimoto K, Sugisaki H, Okamoto T (1976) Sequence of promoter for coat protein gene of bacteriophage fd. Nature 260:297–302Google Scholar
  23. Varshavsky AJ, Nedospasov SA, Bakayev VV, Bakayeva TG, Georgiv GP (1977) Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Res 4:2724–2745Google Scholar
  24. Varshavsky AJ, Bakayev VV, Nedospasov SA, Georgiv GP (1978) On the structure of eukaryotic, prokaryotic and viral chromatin. Cold Spring Harbor Symp Quant Biol 42:457–473Google Scholar
  25. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268Google Scholar
  26. Worcel A, Burgi E (1972) On the structure of the folded chromosome of Escherichia coli. J Mol Biol 71:127–142Google Scholar
  27. Yamazaki K, Nagata A, Kano Y, Imamoto F (1984) Isolation and characterization of nucleoid proteins from Escherichia coli. Mol Gen Genet 196:217–224Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Yasunobu Kano
    • 1
  • Setsuo Yoshino
    • 2
  • Morimasa Wada
    • 1
  • Kazushige Yokoyama
    • 1
  • Masahiro Nobuhara
    • 3
  • Fumio Imamoto
    • 1
  1. 1.Laboratory of Molecular Genetics(Riken) The Institute of Physical and Chemical ResearchWako, Saitama
  2. 2.Central Research LaboratoriesMitsui Toatsu Chemicals, Inc.Yokohama, Kanagawa
  3. 3.Research Laboratories of Cell ScienceMochida Pharmaceutical Co., LTDTokyoJapan

Personalised recommendations