Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 329–333 | Cite as

Weigle reactivation and mutagenesis of bacteriophage λ in lexA(Def) mutants of E. coli K12

  • Patrick Calsou
  • Martine Defais


The SOS response in UV-irradiated bacteria enhances the survival and mutagenesis of infecting damaged bacteriophage λ. In a lexA(Def) strain, SOS bacterial genes are fully derepressed by an inactivating mutation in the LexA repressor gene. We tested several lexA(Def) derivative strains for their capacity to constitutively promote high survival and mutagenesis of irradiated λ. We showed that UV irradiation of the lexA(Def) host bacteria is still necessary for optimal efficiency of both these SOS functions, which are dependent on the umuC gene product and an activated form of RecA protein.


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  1. Bagg A, Kenyon CJ, Walker GC (1981) Inducibility of a gene product required for UV and chemical mutagenesis in E. coli. Proc Natl Acad Sci USA 78:5749–5753Google Scholar
  2. Blanco M, Herrera G, Collado P, Rebello J, Botella IM (1982) Influence of RecA protein on induced mutagenesis. Biochimie 64:633–636Google Scholar
  3. Bridges BA, Woodgate R (1984) Mutagenic repair in E. coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error in UV-mutagenesis. Mol Gen Genet 196:364–366Google Scholar
  4. Caillet-Fauquet P, Maenhaut-Michel G, Radman M (1984) SOS mutator effect in E. coli mutants deficient in mismatch corection. EMBO J 3:707–712Google Scholar
  5. Calsou P, Salles B (1985) Regulation of the SOS response analysed by RecA protein amplification. J Bacteriol 162:1162–1165Google Scholar
  6. Csonka LN, Clark AJ (1979) Deletions generated by the transposon Tn10 in the srl recA region of E. coli K12 chromosome. Genetics 93:321–343Google Scholar
  7. Defais M (1983) Role of the E. coli umuC gene product in the repair of single-stranded DNA phage. Mol Gen Genet 192:509–511Google Scholar
  8. Defais M, Fauquet P, Radman M, Errera M (1971) Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43:495–503Google Scholar
  9. Elledge SJ, Walker GC (1983) The muc genes of pKM101 are induced by DNA damage. J Bacteriol 155:1306–1315Google Scholar
  10. Howard-Flanders P, Boyce RP (1966) DNA repair and genetic recombination: studies on mutants of E. coli defective in these processes. Radiat Res (Suppl) 6:156–184Google Scholar
  11. Howard-Flanders P, Boyce RP, Theriot L (1966) Three loci in E. coli K12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53:1113–1136Google Scholar
  12. Kato T, Shinoura Y (1977) Isolation and characterization of mutants of E. coli deficient in induction of mutations by UV light. Mol Gen Genet 156:121–131Google Scholar
  13. Krueger JH, Elledge SJ, Walker GC (1983) Isolation and characterization of Tn5 insertion mutations in the lexA gene of E. coli. J Bacteriol 153:1368–1378Google Scholar
  14. Little JW, Mount DW (1982) The SOS regulatory system of E. coli. Cell 29:11–22Google Scholar
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  16. Maenhaut-Michel G, Caillet-Fauquet P (1984) The effect of umuC mutations on targeted and untargeted ultraviolet mutagenesis in bacteriophage λ. J Mol Biol 177:181–187Google Scholar
  17. Miller JH (1972) In experiments in molecular genetics. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  18. Morand P, Blanco M, Devoret R (1977) Characterization of lexB mutations in E. coli K12. J Bacteriol 131:572–582Google Scholar
  19. Mount DW (1977) A mutant of E. coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc Natl Acad Sci USA 74:300–304Google Scholar
  20. Paoletti C, Salles B, Giacomoni P (1982) An immunoradiometric quantitative assay of E. coli RecA protein. Biochimie 64:239–246Google Scholar
  21. Quillardet P, Moreau PL, Ginsburg H, Mount DW, Devoret R (1982a) Cell survival, UV-reactivation and induction of prophage lambda in E. coli K12 overproducing RecA protein. Mol Gen Genet 188:37–43Google Scholar
  22. Quillardet P, Huisman O, D'Ari R, Hofnung M (1982b) SOS chromotest, a direct assay of induction of an SOS function in E. coli K12 to measure genotoxicity. Proc Natl Acad Sci USA 79:5971–5975Google Scholar
  23. Radman M (1974) Phenomenology of an inducible mutagenic DNA repair in E. coli: SOS repair hypothesis. In: Prakash L, Sherman F, Miller MW, Lawrence CW, Taber HW (eds) Molecular and environmental aspects of mutagenesis. 6th Rochester Conference on Environmental Toxicity, C.C. Thomas, Springfield II, USA, pp 128–142Google Scholar
  24. Radman (1975) SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. In: Hanawalt PC, Setlow RB (eds) MOlecular mechanisms for repair of DNA. Plenum Press, New York, pp 355–367Google Scholar
  25. Salles B, Paoletti C (1983) Control of UV induction of RecA protein. Proc Natl Acad Sci USA 80:65–69Google Scholar
  26. Thomas R (1966) Control of development in temperate bacteriophage. I. Induction of prophage gene following heteroimmune superinfection. J Mol Biol 22:79–95Google Scholar
  27. Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in E. coli. Microbiol Rev 48:60–93Google Scholar
  28. Weigle JJ (1953) Induction of mutation in a bacterial virus. Proc Natl Acad Sci USA 39:628–636Google Scholar
  29. Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in E. coli. Bacteriol Rev 40:869–967Google Scholar
  30. Witkin EM, Kogoma T (1984) Involvement of the activated form of RecA protein in SOS mutagenesis and stable DNA replication in E. coli. Proc Natl Acad Sci USA 81:7539–7543Google Scholar
  31. Wood RD, Hutchinson F (1984) Non-targeted mutagenesis of unirradiated λ phage in E. coli host cells irradiated with UV light. J Mol Biol 173:293–305Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Patrick Calsou
    • 1
  • Martine Defais
    • 1
  1. 1.Laboratoire de Pharmacologie et de Toxicologie FondamentalesCNRS 205ToulouseFrance

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