Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 204–212 | Cite as

Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids

  • Roy Gross
  • Fred Engelbrecht
  • Volkmar Braun


Iron acquisition via aerobactin enhances the virulence of Escherichia coli. Genes that specify functions for aerobactin synthesis and iron(III)-aerobactin transport have been identified on several ColV plasmids. Previously, we cloned the locus for aerobactin synthesis from pColV-K311 an dassigned to three loci termed AeA, aerB, and areC the functions for hydroxylation of lysine, acetylation of the 6-amino group of 6-hydroxy-lysine and coupling of N-acetyl-N-hydroxy-lysine with citrate, respectively (Gross et al. 1984). In this paper we show that aerA and aerB determine polypeptides with molecular weights of 50,000 and 35,000, respectively. We identified a fourth gene designated aerD that codes for a polypeptide with a molecular weight of 60,000, and which is required for the linkage of one residue of N-acetyl-N-hydroxy-lysine to citrate. The aerC gene product completes aerobactin synthesis by coupling the secod N-acetyl-N-hydroxy-lysine to the monoacylated derivative citrate. The order of the genes in the operon was found to be aerD-aerB-areC-aerA.


Iron Escherichia Coli Molecular Weight Citrate Lysine 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Roy Gross
    • 1
  • Fred Engelbrecht
    • 1
  • Volkmar Braun
    • 1
  1. 1.Mikrobiologie II der UniversitätTübingenGermany

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