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Archives of Microbiology

, Volume 149, Issue 5, pp 406–412 | Cite as

Identification of constitutive and herbicide inducible cytochromes P-450 in Streptomyces griseolus

  • Daniel P. O'Keefe
  • James A. Romesser
  • Kenneth J. Leto
Original Papers

Abstract

The elevated soluble cytochrome P-450 content of Streptomyces griseolus cells found after growth in the presence of sulfonylurea herbicides has been shown to be the result of the appearance of one predominant cytochrome P-450 form. This cytochrome P-450 is the major soluble protein found to increase in amount following herbicide treatment, and functions as part of a sulfometuron methyl hydroxylase system. A second minor inducible cytochrome P-450 has been observed only in cells grown in the presence of chlorimuron ethyl, and a third cytochrome P-450 has been found to be present in all cells independent of the presence of sulfonylurea inducers. The three cytochrome P-450 isozymes are distinguishable primarily by their anion exchange properties; however, spectral properties, substrate inducibility, and enzymatic activity provide several further distinguishing features. The recognition of these inducible, xenobiotic metabolizing cytochromes P-450 in S. griseolus provides the only known description of monooxygenase proteins related to herbicide metabolism in bacteria.

Key words

Cytochrome P-450 Induction Herbicide Detoxification Isozyme Streptomyces griseolus 

Abbreviations

Chlorsulfuron

2-chloro-N-[4-methoxy-6-methyl-1,3,5-triazin-2-yl) aminocarbonyl]benzenesulfonamide

sulfometuron methyl

N-[(4,6-dimethylpyrimidine-2-yl)aminocarbonyl]-2-methoxycarbonyl-benzenesulfonamide

chlorimuron ethyl

N-[(4-chloro-6-methoxypyrimidine-2-yl)aminocarbonyl]2-ethoxycarbonylbenzene-sulfonamide

LDS

lithium dodecyl sulfate

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Daniel P. O'Keefe
    • 1
  • James A. Romesser
    • 1
  • Kenneth J. Leto
    • 2
  1. 1.Central Research and Development DepartmentE. I. du Pont de Nemours and Company, Experimental StationWilmingtonUSA
  2. 2.Agricultural Products DepartmentE. I. du Pont de Nemours and Company, Experimental StationWilmingtonUSA

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