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Molecular and General Genetics MGG

, Volume 200, Issue 2, pp 322–327 | Cite as

Colonization factor antigen II (CFA/II) of enterotoxigenic Escherichia coli: molecular cloning of the CS3 determinant

  • Paul A. Manning
  • Kenneth N. Timmis
  • Gordon Stevenson
Article

Summary

The genes for the cell surface associated antigen CS3, produced by CFA/II type enterotoxigenic Escherichia coli, have been cloned in the plasmid vector pBR322 to produce a family of recombinant plasmids. These plasmids contain a series of HindIII fragments of which a fragment of 4.6 kb is common to all those expressing CS3. One of these plasmids, pPM474, has been subjected to mutagenesis with Tn1725 and deletions generated using Bal31. This has defined a minimum region of 3.75 kb necessary for the production of CS3 on the cell surface and implying genetic complexity as has been observed with other fimbrial antigens. Analysis of the plasmid encoded proteins in E. coli K-12 minicells has confirmed this complexity.

Keywords

Escherichia Coli Cell Surface Molecular Cloning Recombinant Plasmid Plasmid Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Ap

ampicillin

kb

kilobase pairs

R (superscript)

resistant

S (superscript)

sensitive

Tc

tetracycline

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Paul A. Manning
    • 1
  • Kenneth N. Timmis
    • 1
  • Gordon Stevenson
    • 1
  1. 1.Department of Microbiology and ImmunologyThe University of AdelaideAdelaideAustralia

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