Molecular and General Genetics MGG

, Volume 200, Issue 2, pp 229–234 | Cite as

Impaired nitrogen fixation and glutamine synthesis in methionine sulfoximine sensitive (MSs) mutants of Rhizobium phaseoli

  • Enrique Morett
  • Soledad Moreno
  • Guadalupe Espin
Article

Summary

Random Tn5 mutagenesis of antibiotic-resistant derivatives of Rhizobium phaseoli CFN42 yielded several independent mutants that were sensitive to methionine sulfoximine (MSs), a specific inhibitor of glutamine synthetase (GS). These MSs mutants were analyzed for GSI and GSII activities and for their symbiotic properties. Four classes of MSs mutants have been distinguished. Class I strains are impaired in their synthesis of glutamine and in their symbiotic properties. Class II strains have wild type levels of GSI and GSII activities but have a reduced capacity to fix nitrogen. Class III strains have lost GSII activity, but their symbiotic properties are wild type. In class IV mutants neither glutamine synthesis nor symbiotic properties are affected. Mutants of classes I, III, and IV all have the Tn5 inserted into the chromosome, whereas in class II mutants the Tn5 is located in plasmid p42e, a plasmid different from the previously identified symbiotic plasmid p42d.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Enrique Morett
    • 1
  • Soledad Moreno
    • 1
  • Guadalupe Espin
    • 1
  1. 1.Centro de Investigacion sobre Fijacion de NitrogenoUNAMCuernavaca Mor.Mexico

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