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Studies on the growth of Thiobacillus ferrooxidans

IV. Influence of monovalent metal cations on ferrous iron oxidation and uranium toxicity in growing cultures

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Abstract

1. Oxidation of ferrous iron by Thiobacillus ferrooxidans proceeded at the same rates in media grossly deficient in potassium as in media with 4.56 mM K+ added. 2. Iron oxidation in “potassium-free” medium was markedly inhibited by the addition of 10-5 M K+ or Na+ (as sulphates), compared with normal or accelerated oxidation at lower or higher concentrations. 3. Chlorides of sodium or potassium were inhibitory under conditions where the sulphates were not; the concentrations of chlorides required to inhibit development depended on the total potassium content of the medium. 4. Thallium and rubidium were growth inhibitory at 10-4 M in the “potassium-free” medium, but were not toxic at 10-3 M in the normal medium. 5. Inhibition of growth by 2 mM uranyl sulphate was partially relieved by 200 mM K+, Na+, Li+ or NH4 + added to the normal medium as sulphates. 6. Increased H2SO4 concentration increased uranium toxicity without affecting the normal growth rates. 7. The results are discussed in relation to the possible presence in T. ferrooxidans of two K+-transport systems of different reaction to external K+-concentration, and the possible effects of uranium on membrane-dependent processes.

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References

  1. Araki, K., Oka, T., Nakayama, K.: Na+-dependent transport of amino acids and its significance for growth of a lysine-producing bacterium. Agr. Biol. Chem. 37, 1357–1366 (1973)

  2. Armstrong, W. McD., Rothstein, A.: Discrimination between alkali metal cations by yeast. J. gen. Physiol. 48, 61–67 (1964)

  3. Damadian, R.: Biological ion exchanger resins. Ann. N. Y. Acad. Sci. 204, 211–248 (1973)

  4. Drapeau, G. R., Matula, T. I., Macleod, R. A.: Nutrition and metabolism of marine bacteria. J. Bact. 92, 63–71 (1966)

  5. Duncan, D. W., Walden, C. C.: Microbiological leaching in the presence of ferric iron. Devs. ind. Microbiol. 13, 66–75 (1972)

  6. Eisenstadt, E.: Potassium content during growth and sporulation of Bacillus subtilis W23. J. Bact. 112, 264–267 (1972)

  7. Fuhrmann, G.-F., Rothstein, A.: The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim. biophys. Acta (Amst.) 163, 331–338 (1968)

  8. Golomzik, A. I., Ivanov, V. I.: Adaptation of Thiobacillus ferrooxidans to increased hydrogen ion and iron concentrations. Mikrobiologiya 34, 465–468 (1965)

  9. Halpern, Y. S., Barash, H., Dover, S., Druck, K.: Sodium and potassium requirements for active transport of glutamate by Escherichia coli K-12. J. Bact. 114, 53–58 (1973)

  10. Ivarson, K. C.: Microbiological formation of basic ferric sulfates. Canad. J. Soil Sci. 53, 315–323 (1973)

  11. Kamalov, M. R.: Adaptation of Thiobacillus ferrooxidans culture to increased amounts of copper, zinc and molybdenum in acid medium. Izv. Akad. Nauk Kaz. SSR, Ser. Biol. 10, 39–44 (1972)

  12. Leathen, W. W., McIntyre, L. D., Braley, S. A.: A medium for the study of the bacterial oxidation of ferrous iron. Science 114, 280–281 (1951)

  13. Matula, T. I., Sripastava, V. S., Wong, P., Macleod, R. A.: Transport and retention of K+ and other metabolites in a marine pseudomonad and their relation to the mechanism of optical effects. J. Bact. 102, 790–796 (1970)

  14. Oxender, D. J.: Membrane transport. Ann. Rev. Biochem. 41, 777–814 (1972)

  15. Razzell, W. E., Trussell, P. C.: Isolation and properties of an iron-oxidizing Thiobacillus. J. Bact. 85, 595–603 (1963)

  16. Schultz, S. G., Solomon, A. K.: Cation transport in Escherichia coli. J. gen. Physiol. 45, 355–369 (1961)

  17. Silverman, M. P., Lundgren, D. G.: Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J. Bact. 77, 642–647 (1959)

  18. Thompson, J., Macleod, R. A.: Functions of Na+ and K+ in the active transport of α-aminoisobutyric acid in a marine pseudomonad. J. biol. Chem. 246, 4066–4074 (1971)

  19. Tuovinen, O. H., Kelly, D. P.: Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores. Z. allg. Mikrobiol. 12, 311–346 (1972)

  20. Tuovinen, O. H., Kelly, D. P.: Studies on the growth of Thiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with 14CO2-fixation and iron oxidation as measures of growth. Arch. Mikrobiol. 88, 285–298 (1973)

  21. Tuovinen, O. H., Kelly, D. P.: Studies on the growth of Thiobacillus ferrooxidans. II. Toxicity of uranium to growing cultures and tolerance conferred by mutation, other metal cations and EDTA. Arch. Microbiol. 95, 153–164 (1974)

  22. Tuovinen, O. H., Niemelä, S. I., Gyllenberg, H. G.: Tolerance of Thiobacillus ferrooxidans to some metals. Antonie v. Leeuwenhoek 37, 489–496 (1971a)

  23. Tuovinen, O. H., Niemelä, S. I., Gyllenberg, H. G.: Effect of mineral nutrients and organic substances on the development of Thiobacillus ferrooxidans. Biotech. Bioeng. 13, 517–527 (1971b)

  24. Willis, D. B., Ennis, H. L.: Ribonucleic acid and protein synthesis in a mutant of Bacillus subtilis deficient in potassium retention. J. Bact. 96, 2035–2042 (1968)

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Tuovinen, O.H., Kelly, D.P. Studies on the growth of Thiobacillus ferrooxidans . Arch. Microbiol. 98, 167–174 (1974). https://doi.org/10.1007/BF00425279

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Key words

  • Thiobacillus ferrooxidans
  • Iron Oxidation
  • Potassium
  • Monovalent Cations
  • Uranium Toxicity