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Archiv für Mikrobiologie

, Volume 87, Issue 2, pp 173–180 | Cite as

Molecular basis for the differential anti-metabolite action of d-tyrosine in strains 23 and 168 of Bacillus subtilis

  • Roy A. Jensen
  • Sherry L. Stenmark
  • W. Scott Champney
Article

Summary

The basis for the difference between strains 168 (d-tyrosine-sensitive) and 23 (d-tyrosine-resistant) of Bacillus subtilis at the molecular level is that of transport of d-tyrosine into the cell. Strain 23 does not incorporate significant amounts of d-tyrosine into whole cells. A mutant derivative was isolated from strain 23 which had an altered transport system permitting d-tyrosine uptake, a change which also led to inhibition of growth by d-tyrosine. Strain 168 is extremely sensitive to growth inhibition caused by low concentrations of the d-isomer of tyrosine. A mutant derivative of strain 168 selected for its d-tyrosine resistant phenotype had an altered transport system which no longer recognized the d-isomer of tyrosine. These mutants define at least one element of the tyrosine transport system in B. subtilis and provide a convenient phenotype for the eventual location of the chromosal map position.

Keywords

Tyrosine Bacillus Growth Inhibition Molecular Level Transport System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Roy A. Jensen
    • 1
  • Sherry L. Stenmark
    • 1
  • W. Scott Champney
    • 1
  1. 1.Department of MicrobiologyBaylor College of MedicineHoustonUSA

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