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Formation of acetylcarnitine in muscle of horse during high intensity exercise

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Summary

To study the changes in carnitine in muscle with sprint exercise, two Thoroughbred horses performed two treadmill exercise tests. Biopsies of the middle gluteal were taken before, after exercise and after 12 min recovery. Resting mean muscle total carnitine content was 29.5 mmol · kg−1 dry muscle (d. m.). Approximately 88% was free carnitine, 7% acetylcarnitine and acylcarnitine was estimated at 5%. Exercise did not affect total carnitine, but resulted in a marked fall in free carnitine and almost equivalent rise in acetylcarnitine. The results are consistent with a role for carnitine in the regulation of the acetyl-CoA/CoA ratio during sprint exercise in the Thoroughbred horse by buffering excess production of acetyl units.

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References

  1. Alkonyi I, Kerner J, Sandor A (1975) The possible role of carnitine and carnitine acetyl-transferase in the contracting frog skeletal muscle. FEBS Lett 52: 265–268

  2. Bergström J (1962) Muscle electrolytes in man. Determined by neutron activation analysis on needle biopsy specimens. A study on normal subjects, kidney patients, and patients with chronic diahorrea. Scand J Clin Lab Invest 14: Suppl 68

  3. Brass EP, Hoppel CL (1980) Relationship between acid soluble carnitine and coenzyme A pools in vivo. Biochem J 190: 495–504

  4. Bremer J (1983) Carnitine — metabolism and functions. Physiol Rev 63: 1420–1480

  5. Carter AL, Lennon DLF, Stratman FW (1981) Increased acetylcarnitine in rat skeletal muscle as a result of high-intensity short-duration exercise. FEBS Lett 126: 21–24

  6. Cederblad G, Froberg S, Harris RC, Nordesjö LO (1974) Relationship between the turnover rate of plasma free fatty acids, change in intramuscular triglyceride content, and intramuscular content of phospholipid and carnitine. Acta Universitatis Uppsaliensis Medicine: 210

  7. Childress CC, Sacktor B, Traynor DR (1966) Function of carnitine in the fatty acid oxidase-deficient insect flight muscle. J Biol Chem 242: 754–760

  8. Costill DL, Fink WJ, Getchell LH, Ivy JL, Witzmann FA (1979) Lipid metabolism in skeletal muscle of endurance-trained males and females. J Appl Physiol 47: 787–791

  9. Fritz IB (1955) The effect of muscle extracts on the oxidation of palmitic acid by liver slices and homogenates. Acta Physiol Scand 34: 367–385

  10. Garland PB, Randle PJ (1964) Control of pyruvate dehydrogenase in the perfused rat heart by the intracellular concentration of acetyl-isoenzyme A. Biochem J 91: 6c-7c

  11. Harris RC, Hultman E, Nordesjö LO (1974) Glycogen glycolytic intermediates and high energy phosphates in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values. Scand J Clin Lab Invest 33: 109–120

  12. Kerner J, Sandor A (1978) Role of carnitine in skeletal muscle of the rat. Acta Physiol Hung 52: 284

  13. Lennon DLF, Stratman FW, Shrago E, Nagle FJ, Madden M, Hanson P, Carter AL (1983) Effects of acute moderate-intensity exercise on carnitine metabolism in men and women. J Appl Physiol 55: 489–495

  14. Marquis NR, Fritz IB (1964) Enzymological determination of free carnitine concentrations in rat tissue. J Lipid Res 5: 184–187

  15. Pearson DJ, Tubbs PL (1967) Carnitine and its derivatives in rat tissues. Biochem J 105: 953–963

  16. Pearson DJ, Tubbs PK, Chase FA (1974) Carnitine and acylcarnitine. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis, Academic Press, London, pp 1758–1771

  17. Snow DH, Harris RC (1985) Thoroughbreds and Greyhounds: Biochemical adaptations in creatures of nature and of man. In: Gilles R (ed) Circulation, respiration and metabolism. Springer-Verlag, Berlin, pp 227–239

  18. Snow DH, Harris RC, Gash SP (1985) Metabolic response of equine muscle to intermittent maximal exercise. J Appl Physiol 58: 1689–1697

  19. Van Hinsbergh VWM, Veerkamp JH, Glatz JFC (1979) 4-Methyl-2-oxopentanoate oxidation by rat skeletal muscle mitochondria. Biochem J 182: 353–360

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Correspondence to C. V. Louise Foster.

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Foster, C.V.L., Harris, R.C. Formation of acetylcarnitine in muscle of horse during high intensity exercise. Europ. J. Appl. Physiol. 56, 639–642 (1987). https://doi.org/10.1007/BF00424803

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Key words

  • Carnitine
  • Acetylcarnitine
  • Pyruvate metabolism
  • Muscle
  • Horse
  • Exercise