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Isolation and cleanup of organophosphorus insecticides and their oxones from animal tissues

Isolierung und Reinigung von Organophosphorinsecticiden und ihren Oxonen aus tierischen Gewehen

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Zusammenfassung

Ein einfaches Isolations- und Reinigungsverfahren wurde ausgearbeitet. Die Extraktion der Insecticide aus einer Homogenatsäule, die aus Kieselgel und Tiergewebe besteht, umfaßt zum Teil auch die Reinigung der Extrakte, da die polaren Verbindungen an die Säule gebunden bleiben. Der Mittelwert der Ausbeute des Verfahrens wurde im Modellversuch mit 14C- und 32P-markierten Insecticiden zu 90,5% festgestellt. Die Standardabweichung beträgt ± 5,1%.

Summary

A simple isolation and cleanup procedure for the determination of organophosphorus insecticides and their oxones in animal tissues has been developed. This procedure unites partly extraction of organophosphorus pesticides and their oxones with cleanup by column chromatography. Most polar coextracts remain on the column and in its further processing the eluate does not tend to form emulsion. The process has been studied on model mixtures of 32P- and 14C-labelled pesticides with tissues as well as after application of pesticides to white rats. The average recovery of the method is 90.5%, with a standard deviation of ±5.1%.

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References

  1. 1.

    Bátora, V.: Pesticides. In: Paper chromatography, pp. 705–707. I. M. Hais and K. Macek, eds. Prague: Czechoslovak Acad. Sci. 1963.

  2. 2.

    Hladká A., Krampl V., Kováč J., Vladár.M.: Determination of organophosphorus pesticides in animal tissues. Proceedings of the XXVIII. Meeting of the Czechoslovak Chem. Assoc., Pardubice 1972, p. 88.

  3. 3.

    Ivey, M. C., Claborn, H. V.: J. Agr. Food Chem. 19, 1256 (1971).

  4. 4.

    Kojima, K., O'Brien, R. D.: J. Agr. Food Chem. 16, 574 (1968).

  5. 5.

    Kováč J., Bátora, V.: Residue analysis, gas chromatography of multiple organophosphorus insecticides. 3rd Anal. Conference, 24–29 August 1970, Budapest, p. 313.

  6. 6.

    McLeod, H. A., Mulkins, G., Rao, S. L. N.: Bull. Environ. Contam. Toxicol. 4, 224 (1969).

  7. 7.

    Matsumura, F., Ward, C. T.: Arch. Environm. Health 13, 257 (1966).

  8. 8.

    Mills, A. G., Onley, J. H., Gaither, R. A.: J. Assoc. Offic. Agr. Chemists 46, 186 (1963).

  9. 9.

    Schultz, D. R., Marxmiller, R. L., Koos, B. A.: J. Agr. Food Chem. 19, 1238 (1971).

  10. 10.

    Versino, B., van der Venne, M. Th., Vissers, H.: J. Assoc. Offic. Anal. Chemists 54, 147 (1971).

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Hladká, A., Kováč, J. Isolation and cleanup of organophosphorus insecticides and their oxones from animal tissues. fresenius Z Anal Chem 265, 339–342 (1973). https://doi.org/10.1007/BF00423804

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  • Analyse von Insecticiden, phosphorhaltig in Gewebe, tierisches
  • Isolierungs- u. Reinigungsverfahren