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Nickel resistance of Alcaligenes denitrificans strain 4a-2 is chromosomally coded

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A newly isolated aerobic hydrogen-oxidizing bacterium, Alcaligenes denitrificans strain 4a-2, differs from related autotrophic bacteria by containing only a single cytoplasmic, NAD-reducing hydrogenase, and by its high resistance to nickel ions, i.e. tolerance to 20 mM NiCl2. Strain 4a-2 harbors a single plasmid of about 250 kb. On helper-assisted mating of 4a-2 with Alcaligenes eutrophus strains H16,G29, and M85 nickelresistant transconjugants were selected; these did not contain the donor plasmid, however. All three transconjugants tolerated 3 to 10 mM NiCl2. The resistance was constitutively expressed. DNA/DNA hybridization showed homology with EcoRI-digested DNA of the wild type 4a-2 and transconjugants using a DNA probe containing nickel resistance genes of pMOL28. This indicated that the 4a-2 nickel resistance genes are located on the chromosome.

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  1. Aragno M, Schlegel HG (1977) Alcaligenes ruhlandii (Packer and Vishniac) comb nov., a peritriochous hydrogen bacterium previously assigned to Pseudomonas. J Syst Bacteriol 27: 279–281

  2. Bowien B, Schlegel HG (1981) Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annu Rev Microbiol 35: 405–452

  3. Gerstenberg C, Friedrich B, Schlegel HG (1982) Physical evidence for plasmids in autotrophic, especially hydrogen-oxiding bacteria. Arch Microbiol 133: 90–96

  4. Gijsegem Fvan, Toussaint A (1982) Chromosome transfer and r-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis. Plasmid 7: 30–44

  5. Kado CI, Liu ST (1981) Rapid procedure for detection and isolation of large and small plasmids J Bacteriol 145: 1365–1373

  6. Kersters K, DeLey J (1984) Genus Alcaligenes Castellani and Chalmers 1919. In: Krieg NR (ed) Bergey's manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore, pp 361–373

  7. Lejeune P, Mergeay M, Gijsegem Fvan, Faelen F, Gerits J, Toussaint A (1983) Chromosome transfer and R-prime formation mediated by plasmid pULB113 (RP4::mini Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2 J Bacteriol 155: 1015–1026

  8. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

  9. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acids from microorganisms. J Mol Biol 3: 208–218

  10. Mergeay M, Nies D, Schlegel HG, Gerits J, Charles P, Gijsegem Fvan (1985) Alcaligenes eutrophus CH34 is a facultative chomolithotroph with plasmid-bound resistance to heavy metals. J Bacteriol 162: 328–334

  11. Nies A, Nies DH, Silver S (1989) Cloning and expression of plasmid genes encoding resistances to chromate and cobalt in Alcaligenes eutrophus. J Bacteriol 171: 5065–5070

  12. Nies DH, Silver S (1989) Plasmid-determined inducible efflux is responsible for resistance to cadmium, zinc and cobalt in Alcaligenes eutrophus. J Bacteriol 171: 896–900

  13. Schink B, Schlegel HG (1979) The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties. Biochim Biophys Acta 567: 315–324

  14. Schmidt T, Schlegel HG (1989) Nickel and cobalt resistance of various bacteria isolated from soil and highly polluted domestic and industrial wastes. FEMS Microbiol Ecol 62: 315–328

  15. Schneider K, Schlegel HG (1977) Localization and stability of hydrogenases from aerobic hydrogen oxidizing bacteria. Arch Microbiol 112: 229–238

  16. Schneider K, Schlegel HG, Jochim K (1984a) Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b. Eur J Biochem 138: 533–541

  17. Schneider K, Cammack R, Schlegel HG (1984b) Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenases of Nocardia opaca 1 b. Eur J Biochem 142: 75–84

  18. Sensfuss C, Schlegel HG (1988) Plasmid pMOL28-encoded resistance to nickel is due to specific efflux. FEMS Microbiol Lett 55: 295–298

  19. Siddiqui RA, Schlegel HG (1987) Plasmid pMOL28-mediated inducible nickel resistance in Alcaligenes eutrophus strain CH34. FEMS Microbiol Lett 43: 9–13

  20. Siddiqui RA, Schlegel HG, Meyer M (1988) Inducible and constitutive expression of pMOL28-encoded nickel resistance in Alcaligenes eutrophus N9A. J Bacteriol 170: 4188–4193

  21. Siddiqui RA, Benthin K, Schlegel HG (1989) Cloning of pMOL28-encoded nickel resistance genes and expression in Alcaligenes eutrophus and Pseudomonas spec. J Bacteriol 171: 5071–5078

  22. Silver S, Misra TK (1988) Plasmid-mediated heavy metal resistance. Annu Rev Microbiol 42: 717–743

  23. Smith DH (1967) R factors mediate resistance to mercury, nickel and cobalt. Science 156: 1114–1116

  24. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

  25. Timotius K, Schlegel HG (1987) Aus Abwässern isolierte nickel-resistente Bakterien. In: Nachrichten der Akademie der Wissen-schaften in Göttingen 3: 15–23

  26. Warrelmann J, Friedrich B (1989) Genetic transfer of lithoautotrophy mediated by a plasmid-cointegrate from Pseudomonas facilis. Arch Microbiol 151: 359–364

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Kaur, P., Roß, K., Siddiqui, R.A. et al. Nickel resistance of Alcaligenes denitrificans strain 4a-2 is chromosomally coded. Arch. Microbiol. 154, 133–138 (1990).

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Key words

  • Alcaligenes denitrificans
  • Alcaligenes eutrophus
  • Nickel resistance
  • Hydrogenase