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Hemmung des Sialinsäureeinbaus in Ganglioside und Glykoproteine des Kükenhirns durch Chlorpromazin

Effects of chlorpromazine on the incorporation of sialic acid into gangliosides and glycoproteins of the chicken brain

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Abstract

Three-days-old White Leghorn chickens were used to study the effect of i.p.-injected chlorpromazine (CP, 2.5 or 5 mg/animal) on the incorporation of N-(3H)-acetylmannosamine into glycoprotein-and ganglioside-bound sialic acid and 14C-histidine into the proteins of the cerebrum, the optic lobes, and the cerebellum after incorporation periods of 1–24 h. All experiments were performed under constant temperature conditions.

  1. 1.

    The incorporation of 14C-histidine into the proteins of the optic lobes was not influenced by CP.

  2. 2.

    Within 24 h in the optic lobes neither the concentration of particle-bound sialic acid (μg sialic acid/mg protein) nor the content of radioactive-labeled sialic acid precursor was affected by the drug.

  3. 3.

    The rate of incorporation of labeled sialic acid into glycoproteins and gangliosides, however, was up to 6 h after the injection of the precursor much lower in the CP-treated animals than in controls. This may indicate, that the turnover of sialo-compounds was diminished by CP.

  4. 4.

    The inhibition was the most intensive in the cerebrum (up to 80%), lower in the optic lobes (up to 70%), and only small in the cerebellum (up to 20%), but was always the same for glycoproteins and gangliosides.

The different inhibition rates in the different structures may indicate a correlation between the pharmacologic, sedative effects of the drug and the diminished turnover of sialo compounds.

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Rösner, H. Hemmung des Sialinsäureeinbaus in Ganglioside und Glykoproteine des Kükenhirns durch Chlorpromazin. Psychopharmacology 48, 25–29 (1976). https://doi.org/10.1007/BF00423302

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Key words

  • Chloropromazine
  • Glycoproteins
  • Gangliosides
  • Stalic acid