Archives of Microbiology

, Volume 141, Issue 2, pp 164–169 | Cite as

Is organic acid required for nutrition of thermophilic fungi?

  • Sita Devi Gupta
  • Ramesh Maheshwari
Original Papers


In contrast to a published report [Wali et al. Arch Microbiol 118:49–53 (1978)], an organic acid is not essential for the growth of thermophilic fungi. The thermophilic fungus, Thermomyces lanuginosus, grows satisfactorily in a synthetic medium containing glucose as carbon source if the pH of the medium is controlled. The control of pH is essential for the concentration of carbon dioxide in the growth medium and the activity of anaplerotic enzyme, pyruvate carboxylase.

Key words

Thermophilic fungi Growth Organic acid requirement Influence of pH CO2 requirement 





guanosine 5′-diphosphate


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  1. Behrend J, Mateles RI (1976) Nitrogen metabolism in plant cell suspension cultures. II. Role of organic acids during growth on ammonia. Plant Physiol 58:510–512Google Scholar
  2. Budd K (1969) The assimilation of bicarbonate by Neocosmospora vasinfecta. Can J Microbiol 15:389–398Google Scholar
  3. Bushell ME, Bull AT (1981) Anaplerotic metabolism of Aspergillus nidulans and its effect on biomass synthesis in carbon limited chemostats. Arch Microbiol 128:282–287Google Scholar
  4. Butenko RG (1968) Plant tissue culture and morphogenesis. Israel Program for Scientific Translation, JerusalemGoogle Scholar
  5. Charles HP, Broadbent JA (1964) Carbon dioxide mutants: a large and interesting class of Neurospora mutants. Nature 201:1004–1006Google Scholar
  6. Charles HP, Roberts GA (1968) Carbon dioxide as a growth factor for mutants of Escherichia coli. J Gen Microbiol 51:211–224Google Scholar
  7. Cochrane VW (1958) Physiology of fungi. John Wiley, New YorkGoogle Scholar
  8. Conway EJ (1963) Microdiffusion analysis and volumetric error. Chemical Publ Co, New YorkGoogle Scholar
  9. Degryse E, Glansdorff N, Pierard A (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117:189–196Google Scholar
  10. Domsch KH, Gams W, Anderson T-H (1980) Compendium of soil fungi. Academic Press, LondonGoogle Scholar
  11. Gamborg OL, Shyluk JP (1970) The culture of plant cells with ammonium as the sole nitrogen source. Plant Physiol 45:598–600Google Scholar
  12. Grund AD, Ensign JC (1978) Role of carbon dioxide in germination of spores of Streptomyces viridochromogenes. Arch Microbiol 118:279–288Google Scholar
  13. Hartman RE, Keen NT (1973) Enzymes capable of anaplerotic carbon dioxide fixation in Verticillium albo-atrum. Phytopathology 63:947–953Google Scholar
  14. Hartman RE, Keen NT, Long M (1972) Carbon dioxide fixation by Verticillum albo-atrum. J Gen Microbiol 73:29–34Google Scholar
  15. Heinen W (1971) Growth conditions and temperature-dependent substrate specificity of two extremely thermophilic bacteria. Arch Microbiol 76:2–17Google Scholar
  16. Holligan PM, Jennings DH (1972) The influence of different carbon and nitrogen sources on the accumulation of mannitol and arabitol. New Phytol 71:583–594Google Scholar
  17. Krulwich TA, Sharon BI, Perrin LS (1976) Natural paucity of anaplerotic enzymes: Basis for dependence of Arthrobacter pyridinolis on l-malate for growth. J Bacteriol 127:179–183Google Scholar
  18. Lilly VG, Barnett HL (1951) Physiology of the fungi. McGraw-Hill, New York Toronto LondonGoogle Scholar
  19. Loginova LG, Khraptsova GI (1976) Effect of carbon source on development of Thermus ruber at different temperatures. Microbiology 46:29–31 (English translation)Google Scholar
  20. Lowry OH, Rosebrough NG, Farr AL, Randall RJ (1951) Protein estimation with Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  21. McComb RB, Yushok WD (1957) Colorimetric estimation of d-glucose and 2-doexy-d-glucose with glucose oxidase. J Franklin Inst 265:417–422Google Scholar
  22. Morton AG, Macmillan (1954) The assimilation of nitrogen from ammonium salts and nitrate by fungi. J Exp Bot 5:232–252Google Scholar
  23. Ohta Y, Ishikawa M, Abe S, Katoh K, Hirose Y (1981) Growth behaviour of a liverwort, Jungermannia subulata Evans, in a cell suspension culture. The role of organic acids required for cell growth. Plant Cell Physiol 22:1533–1540Google Scholar
  24. Overman SE, Romano AH (1969) Pyruvate carboxylase of Rhizopus nigricans and its role in fumaric acid production. Biochem Biophys Res Commun 37:457–463Google Scholar
  25. Prasad ARS, Maheshwari R (1978) Growth and trehalase activity in the thermophilic fungus Thermomyces lanuginosus. Proc Indian Acad Sci 87B:231–241Google Scholar
  26. Roos W, Luckner M (1984) Relationships between proton extrusion and fluxes of ammonium ions and organic acids in Penicillium cyclopium. J Gen Microbiol 130:1007–1014Google Scholar
  27. Sigler K, Krotkova A, Kotyk A (1981) Factors governing substrate-induced generation and extrusion of protons in the yeast Sacchharomyces cerevisiae. Biochim Biophys Acta 643:572–582Google Scholar
  28. Sobel ME, Wolfson EB, Krulwich TA (1973) Abolition of crypticity of Arthrobacter pyridinolis towards glucose and α-glucosides by tricarboxylic acid cycle intermediate. J Bacteriol 116:271–278Google Scholar
  29. Sutherland IW, Wilkinson JF (1971) Chemical extraction methods of microbial cells. In: Norris JR, Robbins DW (eds) Methods in microbiol, vol 5B. Academic Press, New York, pp 345–383Google Scholar
  30. Walis AS, Mattoo AK, Modi VV (1978) Stimulation of growth and glucose catabolite enzymes by succinate in some thermophilic fungi. Arch Microbiol 118:49–53Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Sita Devi Gupta
    • 1
  • Ramesh Maheshwari
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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