Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Accumulation of proteinase in the cell wall of Streptococcus cremoris strain AM1 and regulation of its production

  • 48 Accesses

  • 29 Citations


The persistent accumulation of proteinase (PIII) activity in the cell wall of Streptococcus cremoris strain AM1 during growth depends on the presence of Ca2+-ions in the medium. In the absence of calcium initial accumulation of activity in the cell wall is observed, followed by a decrease to a low final level. Under this condition no increase of proteolytic activity is found in the extracellular fluid. A possible function of calcium in the stabilization of the enzyme is discussed.

Prolonged accumulation of catalytically active proteinase PIII in the cell wall occurs in the absence of messenger ribonucleic acid synthesis. This process involves de novo protein synthesis supported by preformed proteinase-specific messenger ribonucleic acid, which is possibly either intrinsically long-lived or is stabilized following its transcription. The level of the extracellular concentration of amino acids and/or peptides regulates the translation of newly synthesized proteinase-specific messenger ribonucleic acid and, possibly, the growth of the organism in milk.

This is a preview of subscription content, log in to check access.


  1. Boethling, R. S.: Regulation of extracellular protease secretion in Pseudomonas maltophilia. J. Bacteriol. 123, 954–961 (1975)

  2. Both, G. W., McInnes, J. L., Hanlon, J. E., May, B. K., Elliott, W. H.: Evidence for an accumulation of messenger RNA specific for extracellular protease and its relevance to the mechanism of enzyme secretion in bacteria. J. Mol. Biol. 67, 199–217 (1972)

  3. Coleman, G., Brown, S.: The effect of rifampicin on the stability of the messenger ribonucleic acid of Bacillus amyloliquefaciens as determined by DNA: RNA hybridization. J. Gen. Microbiol. 92, 200–206 (1976)

  4. Efstathiou, J. D., McKay, L. L.: Plasmids in Streptococcus lactis: evidence that lactose metabolism and protease activity are plasmid linked. Appl. Environm. Microbiol. 32, 38–44 (1976)

  5. Exterkate, F. A.: An introductory study of the proteolytic system of Streptococcus cremoris strain HP. Neth. Milk Dairy J 29, 303–318 (1975)

  6. Exterkate, F. A.: Comparison of strains of Streptococcus cremoris for proteolytic activities associated with the cell wall. Neth. Milk Dairy J. 30, 95–105 (1976)

  7. Glenn, A. R., Both, G. W., McInnes, J. L., May, B. K., Elliott, W. H.: Dynamic state of the messenger RNA pool specific for extracellular protease in Bacillus amyloliquefaciens: its relevance to the mechanism of enzyme secretion. J. Mol. Biol. 73, 221–230 (1973)

  8. Glenn, A. R.: Production of extracellular proteins by bacteria. Ann. Rev. Microbiol. 30, 41–62 (1976)

  9. Gould, A. R., May, B. K., Elliott, W. H.: Accumulation of messenger RNA for extracellular enzymes as a general phenomenon in Bacillus amyloliquefaciens. J. Mol. Biol. 73, 213–219 (1973)

  10. Hazen, G. G.: Proteinasen. In: Methoden der Enzymatischen Analyse, 3rd edition (H. U. Bergmeyer, ed.), pp. 1038–1044. Weinheim: Verlag Chemie 1974

  11. Lodish, H. F.: Translational control of protein synthesis. Ann. Rev. Biochem. 45, 39–72 (1976)

  12. McLellan, W. L., Vogel, H. J.: Translational repression in the arginine system of Escherichia coli. Proc. Natl. Acad. Sci. U.S. 67, 1703–1709 (1970)

  13. Priest, F. G.: Effect of glucose and cyclic nucleotides on the transcription of α-amylase mRNA in Bacillus subtilis. Biochem. Biophys. Res. Commun. 63, 606–610 (1975)

  14. Rogosa, M., Franklin, J. C., Perry, K. D.: Correlation of the vitamin requirements with cultural and biochemical characters of Lactobacillus spp. J. Gen. Microbiol. 25, 473–482 (1961)

  15. Semets, E. V., Glenn, A. R., May, B. K., Elliott, W. H.: Accumulation of messenger ribonucleic acid specific for extracellular protease in Bacillus subtilis 168. J. Bacteriol. 116, 531–534 (1973)

  16. Shires, T. K., Pitot, H. C., Kauffman, S. A.: The membron: a functional hypothesis for the translational regulation of genetic expression. Biomembranes 5, 81–145 (1974)

  17. Stinson, M. W., Merrick, J. M.: Extracellular enzyme secretion by Pseudomonas lemoignei. J. Bacteriol. 119, 152–161 (1974)

  18. Stubbs, J. D., Hall, B. D.: Effects of amino acids starvation upon constitutive tryptophan messenger RNA synthesis. J. Mol. Biol. 37, 303–312 (1968)

  19. Thomas, T. D., Jarvis, B. D. W., Skipper, N. A.: Localization of proteinase(s) near the cell surface of Streptococcus lactis. J. Bacteriol. 118, 329–333 (1974)

  20. Venetianer, P.: Level of messenger RNA transcribed from the histidine operon in repressed, depressed and histidine-starved Salmonella typhimurium. J. Mol. Biol. 45, 375–384 (1969)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Exterkate, F.A. Accumulation of proteinase in the cell wall of Streptococcus cremoris strain AM1 and regulation of its production. Arch. Microbiol. 120, 247–254 (1979).

Download citation

Key words

  • Streptococcus cremoris
  • Cell wall proteinase
  • Calcium dependency
  • Regulation
  • Translational control