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Accumulation of proteinase in the cell wall of Streptococcus cremoris strain AM1 and regulation of its production

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Abstract

The persistent accumulation of proteinase (PIII) activity in the cell wall of Streptococcus cremoris strain AM1 during growth depends on the presence of Ca2+-ions in the medium. In the absence of calcium initial accumulation of activity in the cell wall is observed, followed by a decrease to a low final level. Under this condition no increase of proteolytic activity is found in the extracellular fluid. A possible function of calcium in the stabilization of the enzyme is discussed.

Prolonged accumulation of catalytically active proteinase PIII in the cell wall occurs in the absence of messenger ribonucleic acid synthesis. This process involves de novo protein synthesis supported by preformed proteinase-specific messenger ribonucleic acid, which is possibly either intrinsically long-lived or is stabilized following its transcription. The level of the extracellular concentration of amino acids and/or peptides regulates the translation of newly synthesized proteinase-specific messenger ribonucleic acid and, possibly, the growth of the organism in milk.

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Exterkate, F.A. Accumulation of proteinase in the cell wall of Streptococcus cremoris strain AM1 and regulation of its production. Arch. Microbiol. 120, 247–254 (1979). https://doi.org/10.1007/BF00423072

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Key words

  • Streptococcus cremoris
  • Cell wall proteinase
  • Calcium dependency
  • Regulation
  • Translational control