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Diabetologia

, Volume 2, Issue 2, pp 82–85 | Cite as

Free fructose in human cerebrospinal fluid

  • H. Linton Wray
  • Albert I. Winegrad
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Summary

The presence of free fructose in human cerebrospinal fluid has been demonstrated by enzymatic analysis and high voltage paper electrophoresis. In 39 of 40 patients the cerebrospinal fluid fructose concentration exceeded that in plasma. A linear correlation (r = + 0.88) was observed between cerebrospinal fluid fructose and glucose concentrations. Present evidence suggests that neural tissues are the source of cerebrospinal fluid fructose, and that fluctuations in cerebrospinal fluid fructose concentration mirror fluctuations in the neural concentrations of this hexose. These observations suggest that increased activity of the sorbitol pathway may exist in the neural tissues of patients with diabetes mellitus similar to that recently observed in tissue from animals with experimental diabetes.

Keywords

Fructose Sorbitol Hexose Human Cerebrospinal Fluid Free Fructose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Fructose libre dans le liquide cérébro-spinal humain

Résumé

La présence de fructose libre a été démontrée dans le liquide céphalo-rachidien humain par analyse enzymatique et électrophorèse à haut voltage sur papier. Dans 39 cas sur 40 la concentration en fructose du liquide céphalo-rachidien dépassait celle du plasma sanguin. Nous avons observé une corrélation linéaire (r = + 0.88) entre les concentrations de fructose et du glucose dans le liquide céphalo-rachidien. Nos connaissances actuelles suggèrent que le fructose du liquide céphalo-rachidien est produit par les tissus nerveux et que les fluctuations de la concentration de fructose dans ce liquide reflètent les fluctuations de la concentration de cet hexose dans les tissus nerveux. Ces observations nous permettent de croire que la réduction du glucose en sorbitol et son oxydation subséquente en fructose sont plus actives dans le tissu nerveux de malades diabétiques, tout comme cela a été récemment observé pour le tissu nerveux d'animaux atteints de diabète expérimental.

Freie Fructose in der Cerebrospinalflüssigkeit des Menschen

Zusammenfassung

Das Vorhandensein von Fructose im Liquor cerebrospinalis beim Menschen wurde enzymatisch und mit Hochspannungselektrophorese auf Papier nachgewiesen. Die cerebrospinale Fructosekonzentration überstieg in 39 von 40 Fällen diejenige im Blutplasma. Es bestand eine vorzügliche Korrelation (r = + 0.88) zwischen den Konzentrationen von Fructose und Glucose im Liquor. Unsere jetzigen Kenntnisse deuten darauf hin, daß das Nervengewebe den Bildungsort der Liquor-Fructose darstellt und daß Schwankungen in der Konzentration der Fructose im Liquor Schwankungen in der neuralen Konzentration dieser Hexose widerspiegeln. Diese Beobachtungen sind mit der Möglichkeit einer vermehrten Umwandlung von Glucose über Sorbitol zu Fructose im Nervengewebe von Patienten mit Diabetes mellitus vereinbar, ähnlich wie dies bei Tieren mit experimentellem Diabetes beobachtet wurde.

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References

  1. [1]
    Gabbay, K.H., L.O. Merola and R.A. Field: Sorbitol pathway: presence in nerve and cord with substrate accumulation in diabetes. Science 151, 209–210 (1966).Google Scholar
  2. [2]
    Hers, H.G.: Le mécanisme de la formation du fructose seminal et du fructose foetal. Biochem. biophys. Acta 37, 127–138 (1960).Google Scholar
  3. [3]
    Hubbard, R.S., and N.M. Russell: The fructose content of spinal fluid. J. biol. Chem. 119, 647–661 (1937).Google Scholar
  4. [4]
    Kinoshita, J.H., S. Futterman, K. Satoh and L.O. Merola: Factors affecting the formation of sugar alcohols in ocular lens. Biochim. biophys. Acta 74, 340–350 (1963).Google Scholar
  5. [5]
    Kuck, J.: The formation of fructose in the ocular lens. Arch. Ophthal. 65, 840–846 (1961).Google Scholar
  6. [6]
    Klotzsch, H., and H.-U. Bergmeyer: In Methods of Enzymatic Analysis, ed. by H.-U. Bergmeyer. Weinheim/Bergstr., Verlag Chemie, pp. 156–159, 1963.Google Scholar
  7. [7]
    Mabry, C.C., J.D. Gryboski and E.A. Karam: Rapid identification and measurement of mono- and oligosaccharides: An adaptation of high-voltage paper electrophoresis for sugars and its application to biologic materials. J. Lab. clin. Med. 817–830 (Nov. 1963).Google Scholar
  8. [8]
    Mann, T., and U. Parsons: Studie on the metabolism of semen. 6. Role of hormones. Effect of castration, hypophysectomy and diabetes. Relation between blood glucose and seminal fructose. Biochem. J. 46, 440–450 (1950).Google Scholar
  9. [9]
    Park, C.R., L.H. Johnson, J.H. Wright Jr. and H. Batsel: Effect of insulin on transport of several hexoses and pentoses into cells of muscle and brain. Amer. J. Physiol. 191, 13–18 (1957).Google Scholar
  10. [10]
    Roe, J.H.: A colorimetric method for the determination of fructose in blood and urine. J. biol. Chem. 107, 15–22 (1934).Google Scholar
  11. [11]
    Sherman, W.R., and M.A. Stewart: Identification of sorbitol in mammalian nerve. Biochem. and Biophys. Res. Communications 22, 492–497 (1966).Google Scholar
  12. [12]
    Smith, I.: Sugars. In Chromatographic and Electrophoretic Techniques, Vol. I, Chromatography. New York, Interscience Publishers, Inc., pp. 246–260, (1960).Google Scholar
  13. [13]
    Somogyi, M.: A method for the preparation of blood filtrates for the determination of sugar. J. biol. Chem. 86, 655–663 (1930).Google Scholar
  14. [14]
    Stewart, M.A., and J.V. Passonneau: Identification of fructose in mammalian nerve. Biochem. and Biophys. Res. Communications 17, 536–541 (1964).Google Scholar
  15. [15]
    — and O.H. Lowry: Substrate changes in peripheral nerve during ischaemia and Wallerian degeneration. J. Neurochem. 12, 719–727 (1965).Google Scholar
  16. [16]
    Van Heyningen, R.: Formation of polyols by the lens of the rat with “sugar” cataract. Nature 184, 194–195 (1959).Google Scholar

Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • H. Linton Wray
    • 1
  • Albert I. Winegrad
    • 1
  1. 1.George S. Cox Medical Research Institute and the Department of MedicineUniversity of PennsylvaniaPhiladelphia

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