Molecular and General Genetics MGG

, Volume 182, Issue 1, pp 159–163 | Cite as

Analysis of mutations affecting Ty-mediated gene expression in Saccharomyces cerevisiae

  • Michael Ciriacy
  • Valerie M. Williamson
Article

Summary

Yeast translocatable, Ty, elements can cause constitutive synthesis of the glucose-repressible alcohol dehydrogenase (ADHII) when inserted upstream from the 5′ end of the structural gene, ADR2. These insertion mutations, ADR3c, are unstable and give rise to secondary ADHII mutations. The majority of such mutants, adr3, can be attributed to excision of the insertion sequence, leaving behind a single copy of the δ-sequence which occurs as a direct repeat at the ends of the Ty elements. A few adr3 mutants appear to be generated by DNA-rearrangements in the vicinity of the Ty insertion. The occurrence of recessive mutants, tye, which are unlinked to ADR2 indicates that the constitutive expression of ADR2 caused by the Ty insertions requires the function of trans-acting genes. These results support the idea that regulation of Ty-linked ADR2 is actively mediated by the insertion sequence and is probably not due to a mere disruption of the wild-type controlling site.

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References

  1. Bolivar F, Rodriguez RL, Green PJ, Betlach MC, Heynecker HL, Boyer MW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 2: 95–113Google Scholar
  2. Cameron JR, Loh EY, Davis RW (1979) Evidence for transposition of dispersed repetitive DNA families in yeast. Cell 16: 739–751Google Scholar
  3. Ciriacy M (1976) Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae. Mol Gen Genet 145: 327–333Google Scholar
  4. Ciriacy M (1979) Isolation and characterization of further cis- and trans-acting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae. Mol Gen Genet 176: 427–431Google Scholar
  5. Denis CL, Ciriacy M, Young ET (1981) A positive regulatory gene is required for accumulation of functional mRNA for glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J Mol Biol (in press)Google Scholar
  6. Deschamps J, Wiame JM (1979) Mating type effect on cis mutations leading to constitutivity of ornithine transaminase in diploid cells of Saccharomyces cerevisiae. Genetics 92: 749–758Google Scholar
  7. Dubois E, Hiernaux D, Grenson M, Wiame JM (1978) Specific induction of catabolism and its relation to repression of biosynthesis in arginine metabolism of Saccharomyces cerevisiae. J Mol Biol 122: 383–406Google Scholar
  8. Errede B, Cardillo TS, Sherman F, Dubois E, Deschamps J, Wiame JM (1980) Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell 22: 427–436Google Scholar
  9. Farabaugh PJ, Fink GR (1980) Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication. Nature 286: 352–356Google Scholar
  10. Finnegan DJ, Rubin GM, Young MW, Hogness DS (1977) Repeated gene families in Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 42: 1053–1063Google Scholar
  11. Gaffner J, Philippsen P (1980) The yeast transposon Ty1 generates duplication of target DNA on insertion. Nature 286: 414–418Google Scholar
  12. Gross TS, Woods RA (1971) Identification of mutants defective in the first and second steps of de novo purine synthesis in Saccharomyces cerevisiae. Biochim Biophys Acta 247: 13–21Google Scholar
  13. Hartwell LH (1980) Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone. J Cell Biology 85: 811–822Google Scholar
  14. Lemoine Y, Dubois E, Wiame JM (1978) The regulation of urea amidolyase of Saccharomyces cerevisiae. Mating type influence on a constitutivity mutation acting in cis. Mol Gen Genet 166: 251–258Google Scholar
  15. Olson MV, Loughney K, Hall BD (1979) Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci. J Mol Biol 132: 387–410Google Scholar
  16. Roeder GS, Fink GR (1980) DNA rearrangments associated with a transposable element in yeast. Cell 21: 239–249Google Scholar
  17. Rothstein RJ, Sherman F (1980) Dependence on mating type for the overproduction of iso-2-cytochrome c in the yeast mutant CYC7H2. Genetics 94: 891–898Google Scholar
  18. Williamson VM, Bennetzen J, Young ET, Nasmyth K, Hall BD (1980) Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast. Nature 283: 214–216Google Scholar
  19. Williamson VM, Young ET, Ciriacy M (1981) Transposable elements associated with constitutive expression of the yeast alcohol dehydrogenase II. Cell 23: 605–614Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Michael Ciriacy
    • 1
  • Valerie M. Williamson
    • 2
  1. 1.Institut für Mikrobiologie der Technischen Hochschule DarmstadtDarmstadtFederal Republic of Germany
  2. 2.Department of BiochemistryUniversity of WashingtonSeattleUSA

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