Molecular and General Genetics MGG

, Volume 182, Issue 1, pp 159–163 | Cite as

Analysis of mutations affecting Ty-mediated gene expression in Saccharomyces cerevisiae

  • Michael Ciriacy
  • Valerie M. Williamson


Yeast translocatable, Ty, elements can cause constitutive synthesis of the glucose-repressible alcohol dehydrogenase (ADHII) when inserted upstream from the 5′ end of the structural gene, ADR2. These insertion mutations, ADR3c, are unstable and give rise to secondary ADHII mutations. The majority of such mutants, adr3, can be attributed to excision of the insertion sequence, leaving behind a single copy of the δ-sequence which occurs as a direct repeat at the ends of the Ty elements. A few adr3 mutants appear to be generated by DNA-rearrangements in the vicinity of the Ty insertion. The occurrence of recessive mutants, tye, which are unlinked to ADR2 indicates that the constitutive expression of ADR2 caused by the Ty insertions requires the function of trans-acting genes. These results support the idea that regulation of Ty-linked ADR2 is actively mediated by the insertion sequence and is probably not due to a mere disruption of the wild-type controlling site.


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Michael Ciriacy
    • 1
  • Valerie M. Williamson
    • 2
  1. 1.Institut für Mikrobiologie der Technischen Hochschule DarmstadtDarmstadtFederal Republic of Germany
  2. 2.Department of BiochemistryUniversity of WashingtonSeattleUSA

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