Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Immuno-fluorescent reactivity to anti-somatostatin in the gastro-intestinal mucosa of the dog

  • 70 Accesses

  • 60 Citations

Summary

Evidence is presented that somatostatin-containing cells are present in the gastro-intestinal tract of the dog. Thus immuno-fluorescent cells were detected by the use of antiserum to cyclic somatostatin. These cells were mainly encountered in the antral mucosa and in the neighbourhood of gastrin-producing cells. No cross reaction was observed between gastrin and somatostatin. It is suggested that locally produced somatostatin controls gastrin secretion, and, more generally, that somatostatin-containing cells, multifocally distributed, modulates secretion of a large number of glands.

References

  1. 1.

    Brazeau, P., Vale, W., Burgus, R., Ling, N., Butcher, M., Rivier, J., Guillemin, R.: Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 179, 77–79 (1973)

  2. 2.

    Alberti, K.G.M.M., Christensen, S.E., Iversen, J., Seyer-Hansen, K., Christensen, N.J., Hensen, A.P., Lundbaeck, K., Orskov, H.: Inhibition of insulin secretion by somatostatin. Lancet 1973 II, 1299–1301

  3. 3.

    Koerker, D.J., Ruch, W., Chideckel, E., Palmer, J., Goodner, C.J. Ensinck, J., Gale, C.C.: Somatostatin: hypothalamic inhibitor of the endocrine pancreas. Science 184, 482–484 (1974)

  4. 4.

    De Vane, G.W., Siler, T.M., Yen, S.S.C.: Acute suppression of insulin and glucose levels by synthetic somatostatin in normal human subjects. J. clin. Endocr. 38, 913–915 (1974)

  5. 5.

    Yen, S.S.C., Siler, T.M., De Vane, G.W.: Effect of somatostatin in patients with acromegaly. Suppression of growth hormone, prolactin, insulin and glucose levels. New Engl. J. Med. 290, 935–938 (1974)

  6. 6.

    Mortimer, C.H., Carr, D., Lind, T., Bloom, S.R., Mallinson, C.N., Schally, A.V., Turnbridge, W.G.M., Yeomans, L., Coy, D.H., Kastin, A., Besser, G.M., Hall, R.: Effects of growth-hormone release-inhibiting hormone on circulating glucagon, insulin, and growth hormone in normal, diabetic, acromegalic, and hypo-pituitary patients. Lancet 1974 I, 697–701

  7. 7.

    Chen, M., Smith, P.H., Woods, S.C., Johnson, D.G., Porte, D., Jr.: Direct inhibitory effects of somatostatin on dog pancreatic endocrine function in vivo. Diabetes 23, 356 (1974)

  8. 8.

    Sakurai, H., Unger, R.: Effects of somatostatin (SRIF) on insulin (I) and glucagon (G) and I/G ratio in normal and diabetic dogs. Diabetes 23, 356 (1974)

  9. 9.

    Gerich, J., Lorenzi, M., Schneider, V., Kwan, C., Karam, J., Forsham, P.H.: Inhibition of pancreatic glucagon responses to arginine by somatostatin in normal subjects and in insulin dependent diabetics. Diabetes 23, 356 (1974)

  10. 10.

    Efendic, S., Luft, R., Grill, V.: Effect of somatostatin on glucose induced insulin release in isolated perfused rat pancreas and isolated rat pancreatic islets. FEBS Letters 42, 169–172 (1974)

  11. 11.

    Iversen, J.: Inhibition of pancreatic glucagon release by somatostatin in vitro. Scand. J. clin. Lab. Invest. 33, 125–129 (1974)

  12. 12.

    Curry, D.L., Bennett, L.L., Li, C.H.: Direct inhibition of insulin secretion by synthetic somatostatin. Biochem. biophys. Res. Commun. 58, 885–889 (1974)

  13. 13.

    Fujimoto, W.Y., Ensinck, J.W., Williams, R.H.: Somatostatin inhibits insulin and glucagon release by monolayer cell cultures of rat endocrine pancreas. Life Sci. 15, 1999–2004 (1974)

  14. 14.

    Dobbs, R., Sakurai, H., Sasaki, H., Faloona, G., Valverde, I., Baetens, D., Orci, L., Unger, R.: Glucagon: role in hyperglycemia of diabetes mellitus. Science 187, 544–547 (1975)

  15. 15.

    Siler, T.M., Yen, S.S.Y., Vale, W., Guillemin, R.: Inhibition by somatostatin on the release of TSH induced in man by thyrotropin-releasing factor. J. clin. Endocr. 38, 742–745 (1974)

  16. 16.

    Arnold, R., Köbberling, J., Track, N. S., Creutzfeldt, W.: Lowering of basal and stimulated serum immunoreactive gastrin and gastric secretion in patients with Zollinger-Ellison syndrome by somatostatin. Acta endocr. (Kbh.) suppl. 193, 75 (1975)

  17. 17.

    Hökfelt, T., Efendic, S., Johansson, O., Luft, R., Arimura, A.: Immunohistochemical localization of somatostatin (growth hormone release-inhibiting factor) in the guinea pig brain. Brain Res. 80, 165–169 (1974)

  18. 18.

    Pelletier, G., Larbie, F., Arimura, A., Schally, A.V.: Electron microscopic immunohistochemical localization of growth hormone-release inhibiting hormone (somatostatin) in the rat median eminence. Amer. J. Anat. 140, 445–450 (1974)

  19. 19.

    Dubois, M.P., Barry, J., Leonardelli, J.: Mise en évidence par immunofluorescence et répartition de la somatostatine (SRIF) dans l'éminence médiane des Vertébrés (mammifères, oiseaux, amphibiens, poissons). C. R. Acad. Sci. (Paris) 279, 1899–1902 (1974)

  20. 20.

    Luft, R., Efendic, S., Hökfelt, T., Johansson, O., Arimura, A.: Immunohistochemical evidence for the localization of somatostatin-like immunoreactivity in a cell population of the pancreatic islets. Medical Biology 52, 428–430 (1974)

  21. 21.

    Dubois, M.P.: Presence of immunoreactive somatostatin in discrete cells of the endocrine pancreas. Proc. nat. Acad. Sci. (Wash.) 70, 1340–1343 (1975)

  22. 22.

    Arimura, A., Sato, H., DuPont, A., Nishi, N., Schally, A.V.: Abundance of immunoreactive GH-release inhibiting hormone in the stomach and the pancreas of rat. Fed. Proc. 34, 273 (1975)

  23. 23.

    Lostra, F., van der Loo, W., Gepts, W.: Are gastrin-cells present in mammalian pancreatic islets? Diabetologia 10, 291–302 (1974)

  24. 24.

    Coons, A.H., Leduc, E.H., Connolly, J.M.: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. exp. Med. 102, 49–63 (1955)

  25. 25.

    Arnold, R., Creutzfeldt, W.: Hemmung der pentagastrininduzierten Säuresekretion des Magens beim Menschen durch Somatostatin. Dtsch. med. Wschr. 100, 1014–1016 (1975)

  26. 26.

    Barros D'Sa, A.A.J., Bloom, S.R., Baron, J.H.: Direct inhibition of gastric acid by growth-hormone release-inhibiting hormone in dogs. Lancet 1975 1, 886–887

  27. 27.

    Gomez-Pan, A., Reed, J.D., Albinus, M., Shaw, B., Hall, R.: Direct inhibition of gastric acid and pepsin secretion by growth hormone release-inhibiting hormone in cats. Lancet 1975 I, 888–890

  28. 28.

    Creutzfeldt, W., Lankisch, P.G., Fölsch, U.R.: Hemmung der Sekretin- und Cholezystokinin-Pankreozymininduzierten Saftund Enzymsekretion des Pankreas und der Gallenblasenkontraktion beim Menschen durch Somatostatin. Dtsch. med. Wschr. 100, 1135–1138 (1975)

  29. 29.

    Fujita, T., Kobayashi, S.: The cells and hormones of the GEP endocrine system. In: Gastro-entero-pancreatic endocrine system (ed. T. Fujita), p. 1. Stuttgart: Georg Thieme 1973

  30. 30.

    Roth, A.: Quantitative studies on the islets of Langerhans in the pigeon. Acta anat. (Basel) 69, 609–622 (1968)

  31. 31.

    Orci, L., Baetens, D., Rufener, C.: Evidence for the D-cell of the pancreas secreting somatostatin. Horm. Metab. Res. (in press)

  32. 32.

    Rufener, C., Amherdt, M., Dubois, M. P., Orci, L.: Ultrastructural immunocytochemical localization of somatostatin in D-cells of rat pancreatic monolayer culture, (submitted for publication)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rufener, C., Dubois, M.P., Malaisse-Lagae, F. et al. Immuno-fluorescent reactivity to anti-somatostatin in the gastro-intestinal mucosa of the dog. Diabetologia 11, 321–324 (1975). https://doi.org/10.1007/BF00422398

Download citation

Key words

  • Immuno-fluorescence
  • gastro-intestinal tract
  • somatostatin
  • gastrin