, Volume 11, Issue 4, pp 307–312 | Cite as

Platelet hyperaggregation and increased plasma level of von Willebrand factor in diabetics with retinopathy

  • D. Bensoussan
  • S. Levy-Toledano
  • P. Passa
  • J. Caen
  • J. Canivet


In 18 insulin-dependent diabetics (6 without retinopathy, 6 with proliferative retinopathy and 6 with proliferative retinopathy treated by hypophysectomy) matched for age and duration of diabetes, in vitro haemostasis was studied using ADP induced platelet aggregation, ristocetin induced platelet aggregation which allows von Willebrand factor (VIII VWF) assay, and determination of antihemophilic factor procoagulant activity (VIII AHF). Using gel filtration-isolated platelets, the ADP induced hyperaggregation previously reported in diabetics with severe retinopathy untreated by hypophysectomy appeared to be related to a platelet and not a plasma factor; the normal results of thrombin induced aggregation suggests that the presumed abnormal platelet factor is related to the platelet plasma membrane. High level of plasma VIII VWF was observed in diabetics with proliferative retinopathy while the VIII AHF level was within normal limits.

Key words

Diabetes retinopathy hypophysectomy ADP induced platelet aggregation ristocetin induced platelet aggregation Von Willebrand factor antihemophilic procoagulant factor 

Abbrevations used in this paper


platelet rich plasma


platelet poor plasma


gel filtered platelets


Von Willebrand factor associated with factor VIII molecule


antihemophilic factor procoagulant activity


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • D. Bensoussan
    • 1
  • S. Levy-Toledano
    • 1
  • P. Passa
    • 1
  • J. Caen
    • 1
  • J. Canivet
    • 1
  1. 1.Dept. of Endocrinology and of Haemostasis and Experimental ThrombosisUniversity Paris-VII, Hôpital Saint LouisParisFrance

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