Advertisement

Diabetologia

, Volume 11, Issue 4, pp 307–312 | Cite as

Platelet hyperaggregation and increased plasma level of von Willebrand factor in diabetics with retinopathy

  • D. Bensoussan
  • S. Levy-Toledano
  • P. Passa
  • J. Caen
  • J. Canivet
Originals

Summary

In 18 insulin-dependent diabetics (6 without retinopathy, 6 with proliferative retinopathy and 6 with proliferative retinopathy treated by hypophysectomy) matched for age and duration of diabetes, in vitro haemostasis was studied using ADP induced platelet aggregation, ristocetin induced platelet aggregation which allows von Willebrand factor (VIII VWF) assay, and determination of antihemophilic factor procoagulant activity (VIII AHF). Using gel filtration-isolated platelets, the ADP induced hyperaggregation previously reported in diabetics with severe retinopathy untreated by hypophysectomy appeared to be related to a platelet and not a plasma factor; the normal results of thrombin induced aggregation suggests that the presumed abnormal platelet factor is related to the platelet plasma membrane. High level of plasma VIII VWF was observed in diabetics with proliferative retinopathy while the VIII AHF level was within normal limits.

Key words

Diabetes retinopathy hypophysectomy ADP induced platelet aggregation ristocetin induced platelet aggregation Von Willebrand factor antihemophilic procoagulant factor 

Abbrevations used in this paper

PRP

platelet rich plasma

PPP

platelet poor plasma

GFP

gel filtered platelets

VWF or VIII VWF

Von Willebrand factor associated with factor VIII molecule

AHF or VIII AHF

antihemophilic factor procoagulant activity

References

  1. 1.
    Heath, H., Brigden, W.D., Canever, J.V., Pollock, J., Hunter, P.R., Kelsey, J., Bloom, A.: Platelet adhesiveness and aggregation in relation to diabetic retinopathy. Diabetologia 7, 308–315(1971)Google Scholar
  2. 2.
    Kwaan, H.C., Colwell, J.A., Cruz, S., Suwanwela, N., Dobbie, J.G.: Increased platelet aggregation in diabetes mellitus. J. Lab. clin. Med. 80, 236–246 (1972)Google Scholar
  3. 3.
    Passa, P., Bensoussan, D., Levy-Toledano, S., Caen, J., Canivet, J.: Etude de l'agrégation plaquettaire au cours de la rétinopathie diabétique. Influence de l'hypophysectomie. Atherosclerosis 19, 277–285 (1974)Google Scholar
  4. 4.
    Howard, M.A., Firkin, B.G.: Ristocetin — a new tool in the investigation of platelet aggregation. Thrombos. Diathes. haemorrh. (Stuttg.) 26, 362–369 (1971)Google Scholar
  5. 5.
    Weiss, H.J., Rogers, J., Brand, H.: Defectiveristocetin-induced platelet aggregation in Von Willebrand's disease and its correction by factor VIII. J. clin. Invest. 52, 2697–2707 (1973)Google Scholar
  6. 6.
    Weiss, H.J., Hoyer, L.W., Rickles, F.R., Varma, A., Rogers, J.: Quantitative assay of a plasma factor deficient in Von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content. J. clin. Invest. 52, 2708–2716 (1973)Google Scholar
  7. 7.
    Levy-Toledano, S., Rendu, F., Besson, P., Caen, J.: Aggregation of human gel filtered platelets. Requirement of apyrase and proteins. Rev. Europ. Et. Clin. Biol. 5, 513–518 (1972)Google Scholar
  8. 8.
    Tangen, O., Berman, H.J., Marfey, P.: Gel filtration. A new technique for separation of blood platelets from plasma. Thrombos. Diathes. haemorrh. (Stuttg.) 25, 268–278 (1971)Google Scholar
  9. 9.
    Born, G.V.R.: Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (Lond.) 194, 927–929 (1962)Google Scholar
  10. 10.
    Soulier, J.P., Larrieu, M.J.: Nouvelle méthode de diagnostic de l'hémophilie. Dosage des facteurs antihémophiliques A et B. Le Sang 24, 205–215 (1953)Google Scholar
  11. 11.
    Leone, G., Bizzi, B., Accoria, F., Boni, P.: Functional aspect of platelets in diabetes mellitus. In: Platelet aggregation and drugs (ed. L. Caprino, F.C. Rossi), pp. 49–61. New York: Acad. Press publish. 1974Google Scholar
  12. 12.
    Metcalf, L.C., Lyman, D.J.: The effect of conformational changes on the blood platelet reactivity of polylysine. Thrombosis Res. 5, 709–717 (1974)Google Scholar
  13. 13.
    Nurden, A.T., Caen, J.P.: An abnormal glycoprotein pattern in three cases of Glanzmann thrombasthenia. Brit. J. Haemal. 28, 255–262 (1974)Google Scholar
  14. 14.
    Beisswenger, P.J., Spiro, R.G.: Human glomerular basement membrane: chemical alteration in diabetes mellitus. Science 168, 596–598 (1970)Google Scholar
  15. 15.
    Odegaard, A.E., Skalhegg, B.A., Hellem, A.J.: Increased activity of “Anti Willebrand factor” in diabetic plasma. Thrombos. Diathes. haemorrh. (Stuttg.) 11, 27–37, (1964)Google Scholar
  16. 16.
    Egeberg, O.: The blood coagulability in diabetic patients. Scand. J. clin. Lab. Invest. 15, 533–538 (1963)Google Scholar
  17. 17.
    Mayne, E.E., Bridges, J.M., Weaver, J.A.: Platelet adhesiveness, plasma fibrinogen and factor VIII levels in diabetes mellitus. Diabetologia 6, 436–440 (1970)Google Scholar
  18. 18.
    Van Mourik, K.A., Bouma, B.N., Labruyere, W.T., De Graal, S., Mochtar, I.A.: Factor VIII, series of homologous oligomers and a complex of two proteins. Thrombosis Res. 4, 155–164 (1974)Google Scholar
  19. 19.
    Hoyer, L.W., De Los Santos, R.P., Hoyer, J.R.: Antihemophilic factor antigen. Localisation in endothelial cells by immunofluorescence in microscopy. J. clin. Invest. 52, 2737–2744 (1973)Google Scholar
  20. 20.
    Jaffe, E.A., Hoyer, L.W., Nachman, R.L.: Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J. clin, Invest. 52, 2757–2764 (1973)Google Scholar
  21. 21.
    Caen, J., Levy-Toledano, S.: Interaction between platelets and Von Willebrand factor provides a new scheme for primary haemostasis. Nature New Biology 244, 159–160 (1973)Google Scholar
  22. 22.
    Kohner, E.N., Dollery, C.T., Paterson, J.W., Oakley, N.W., Fraser, T.R.: Arterial fluorescein studies in diabetic retinopathy. Diabetes 16, 1–10 (1967)Google Scholar
  23. 23.
    Caird, F.I., Pirie, A., Ramsell, T.G.: Diabetes and the eye, p. 24. Oxford, Blackwell: 1969Google Scholar
  24. 24.
    Bloodworth, J.M.B., Jr, Engerman, R.L., Davis, M.D.: Pathology of diabetic microangiopathy. In: Diabetes, Proc. 7th Congr. Intern. Diabetes Fed. (eds. R.R. Rodriguez, J. Vallance-Owen), pp. 804–819. Amsterdam: Excerpta Medica 1971Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • D. Bensoussan
    • 1
  • S. Levy-Toledano
    • 1
  • P. Passa
    • 1
  • J. Caen
    • 1
  • J. Canivet
    • 1
  1. 1.Dept. of Endocrinology and of Haemostasis and Experimental ThrombosisUniversity Paris-VII, Hôpital Saint LouisParisFrance

Personalised recommendations