Antonie van Leeuwenhoek

, Volume 46, Issue 4, pp 321–329 | Cite as

Virulence of Escherichia coli in acute pyelonephritis, acute cystitis and asymptomatic bacteriuria

  • J. F. van den Bosch
  • P. L. Oe
  • P. Postma
  • J. de Graaff
  • D. M. MacLaren
  • W. H. Jansen
  • P. A. M. Guinée
Medical Microbiology
Received:

Abstract

E. coli strains were isolated from urine specimens of hospitalised patients with acute pyelonephritis, acute cystitis or asymptomatic bacteriuria (ABU), and tested for virulence in an experimental mouse model. Of 12 pyelonephritisstrains 11 were shown to be virulent and 1 avirulent; of 12 cystitis-strains 4 were virulent and 8 avirulent; of 12 ABU-strains 5 were virulent and 7 avirulent. It is concluded that, while no difference in virulence was found between cystitis-and ABU-strains, pyelonephritis-strains were more often virulent than cystitis-and ABU-strains.

No associations could be shown between virulence of the isolated strains and the presence of antibody-coated bacteria in the urine. Common urinary O types were not more often virulent than other O types. No relationship was seen between virulence and the presence of K antigen or the presence of particular K types.

Keywords

Mouse Model Cystitis Pyelonephritis Hospitalise Patient Bacteriuria 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Braude, R. and Block, C. 1977. Proteinuria and antibody-coated bacteria in the urine.—N. Engl. J. Med. 297: 617–618.Google Scholar
  2. Dootson, P. H., MacLaren, D. M. and Titcombe, D. H. M. 1973 The distribution of urinary O-groups of Escherichia coli in urinary infections and in the normal faecal flora. p. 139–145. In W. Brumfitt and A. W. Asscher (eds), Urinary tract infection.—Oxford University Press, London.Google Scholar
  3. Fang, L. S. T., Tolkoff-Rubin, N. E. and Rubin, R. H. 1978. Efficacy of single-dose and conventional amoxicillin therapy in urinary-tract infection localized by the antibody-coated bacteria technique. — N. Engl. J. Med. 298: 413–416.Google Scholar
  4. Favaro, S., Conventi, L., Baggio, B., Antonello, A., Zanetti, M., Zanon, G. F., Picchi, A., Fagiolo, U. and Borsatti, A. 1978. Antibody-coated bacteria in the urinary sediments of rats with experimental pyelonephritis. — Nephron 21: 165–169.Google Scholar
  5. Forsum, U., Fritjofsson, A., Frõdin, L., Hjelm, E. and Jonsell, G. 1978. A clinical evaluation of a test for antibody-coated bacteria in the urine. — Scand. J. Urol. Nephrol. 12: 45–48.Google Scholar
  6. Freedman, R. L. 1979. Interstitial renal inflammation, including pyelonephritis and urinary tract infection. p. 850–852. In L. E. Early and C. W. Gottschalk (eds), Strauss and Welt's diseases of the kidney, Vol II, 3rd Ed. — Little Brown and Company, Boston.Google Scholar
  7. Glynn, A. A., Brumfitt, W. and Howard, C. J. 1971. K antigens of Escherichia coli and renal involvement in urinary-tract infections. — Lancet 1971, 1: 514–516.Google Scholar
  8. Goodman, L. A. 1970. The multivariate analysis of qualitative data: Interactions among multiple classifications. — J. Am. Stat. Assoc. 65: 226–256.Google Scholar
  9. Grüneberg, R. N., Leigh, D. A. and Brumfitt, W. 1968. Escherichia coli serotypes in urinary tract infection: studies in domiciliary, antenatal and hospital practice. p. 68–79. In F. O'Grady and W. Brumfitt (eds), Urinary tract infection. — Oxford University Press, London.Google Scholar
  10. Grüneberg, R. N. and Bettelheim, 1969. Geographical variation in serological types of urinary Escherichia coli. — J. Med. Microbiol. 2: 219–224.Google Scholar
  11. Guinée, P. A. M., Agterberg, C. M. and Jansen, W. H. 1972. Escherichia coli O antigen typing by means of a mechanized microtechnique. — Appl. Microbiol. 24: 127–131.Google Scholar
  12. Harding, G. K. M., Marrie, T. J., Ronald, A. R., Hoban, S. and Muir, P. 1978. Urinary tract infection localization in women. — J. Am. Med. Assoc. 240: 1147–1150.Google Scholar
  13. Harle, E. M. J., Bullen, J. J. and Thomson, D. A. 1975. Influence of oestrogen on experimental pyelonephritis caused by Escherichia coli. — Lancet 1975, 2: 283–286.Google Scholar
  14. Kaijser, B., Hanson, L. A., Jodal, U., Lidin-Janson, G. and Robbins, J. B. 1977. Frequency of E. coli K antigens in urinary-tract infections in children. — Lancet 1977, 1: 663–664.Google Scholar
  15. Kalmanson, G. M., Harwick, H. J., Turck, M. and Guze, L. B. 1975. Urinary-tract infection: localisation and virulence of Escherichia coli. — Lancet 1975, 1: 134–136.Google Scholar
  16. Kennedy, R. P., Plorde, J. J. and Petersdorf, R. G. 1965. Studies on the epidemiology of Escherichia coli infections. IV. Evidence for a nosocomial flora. — J. Clin. Invest. 44: 193–201.Google Scholar
  17. Kwasnik, I., Klauber, G. and Tilton, R. C. 1979. Clinical and laboratory evaluation of the antibody-coated bacteria test in children. — J. Urol. 121: 658–661.Google Scholar
  18. Lindberg, U., Hanson, L. A., Jadal, U., Lidin-Janson, G., Lincoln, K. and Olling, S. 1975. Asymptomatic bacteriuria in schoolgirls. II. Differences in Escherichia coli causing asymptomatic and symptomatic bacteriuria. — Acta Paediatr. Scand. 64: 432–436.Google Scholar
  19. Mundt, K. A. and Polk, B. F. 1979. Identification of site of urinary-tract infections by antibody-coated bacteria assay. — Lancet 1979, 2: 1172–1175.Google Scholar
  20. Ørskov, I., Ørskov, F., Jann, B. and Jann, K. 1977. Serology, chemistry and genetics of O and K antigens of Escherichia coli. — Bacteriol. Rev. 41: 667–710.Google Scholar
  21. Peddie, B. A. and Little, P. J. 1978. Serogroups of Escherichia coli in symptomatic and asymptomatic urinary tract infections in Christchurch. — Zbl. Bakt. Hyg., I Abt. Orig. A 240: 320–325.Google Scholar
  22. Rumans, L. W. and Vosti, K. L. 1978. The relationship of antibody-coated bacteria to clinical syndromes. — Arch. Intern. Med. 138: 1077–1081.Google Scholar
  23. Thomas, V., Shelekov, A. and Forland, M. 1974. Antibody-coated bacteria in the urine and the site of urinary-tract infection. — N. Engl. J. Med. 290: 588–590.Google Scholar
  24. Van den Bosch, J. F., De Graaff, J. and MacLaren, D. M. 1979. Virulence of Escherichia coli in experimental hematogenous pyelonephritis in mice. — Infect. Immun. 25: 68–74.Google Scholar
  25. Wong, W. T. and Bettelheim, K. A. 1976. Serotypes of Escherichia coli from urinary tract infections in Hong Kong. — Zbl. Bakt. Hyg., I Abt. Orig. A 236: 481–486.Google Scholar

Copyright information

© H. Veenman en Zonen B.V. 1980

Authors and Affiliations

  • J. F. van den Bosch
    • 1
    • 2
    • 3
  • P. L. Oe
    • 1
    • 2
    • 3
  • P. Postma
    • 1
    • 2
    • 3
  • J. de Graaff
    • 1
    • 2
    • 3
  • D. M. MacLaren
    • 1
    • 2
    • 3
  • W. H. Jansen
    • 4
  • P. A. M. Guinée
    • 4
  1. 1.Department of Medical MicrobiologyFree UniversityAmsterdamThe Netherlands
  2. 2.Department of Oral MicrobiologyFree UniversityAmsterdamThe Netherlands
  3. 3.Department of NephrologyFree UniversityAmsterdamThe Netherlands
  4. 4.National Institute of Public HealthBilthovenThe Netherlands

Personalised recommendations