Several regions of the pentobarbital-anesthetized dog brain of less than 1 mm3 in volume were perfused using a modified Gaddum push-pull cannula. Tryptamine was recovered from perfusates of the cerebral cortex, hippocampus, caudate, thalamus and hypothalamus and was released at a rate of about 0.1 ng/min. The mean blood concentration of tryptamine was approximately 7 ng/ml. Isocarboxazid pretreatment did not increase the amount of tryptamine in the perfusate.
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Martin, W.R., Sloan, J.W., Buchwald, W.F. et al. The demonstration of tryptamine in regional perfusates of the dog brain. Psychopharmacologia 37, 189–198 (1974). https://doi.org/10.1007/BF00421532