Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The demonstration of tryptamine in regional perfusates of the dog brain

  • 17 Accesses

  • 19 Citations

Abstract

Several regions of the pentobarbital-anesthetized dog brain of less than 1 mm3 in volume were perfused using a modified Gaddum push-pull cannula. Tryptamine was recovered from perfusates of the cerebral cortex, hippocampus, caudate, thalamus and hypothalamus and was released at a rate of about 0.1 ng/min. The mean blood concentration of tryptamine was approximately 7 ng/ml. Isocarboxazid pretreatment did not increase the amount of tryptamine in the perfusate.

This is a preview of subscription content, log in to check access.

References

  1. Bell, J. A., Martin, W. R.: The effect in the cat of tryptamine (T) and LSD on cutaneous C-fiber (CFR) and polysynaptic (PSR) spinal reflexes. Pharmacologist 15, 161 (1973)

  2. Frankhauser, R.: Der Liquor oerebrospinalis in der VeterinÄrmedizin. Zentbl. Vet. Med. 1, 136–159 (1953) (cf., McGrath, J. T.: Neurologic examination of the dog with clinicopathologic observations. Philadelphia: Lea & Febiger 1956)

  3. Hess, S. M., Udenfriend, S.: A fluorometric procedure for the measurement of tryptamine in tissues. J. Pharmacol. exp. Ther. 127, 175–177 (1959)

  4. Kluver, H., Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Neuropath. exp. Neurol. 12, 400–403 (1953)

  5. Lilly, J. C.: Electrode and cannulae implantation in the brain by a simple percutaneous method. Science 127, 1181–1182 (1958)

  6. Lim, R. K. S., Liu, C.-N., Moffitt, R. L.: A stereotaxic atlas of the dog's brain. Springfield, Ill.: Ch. C. Thomas 1960

  7. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

  8. Martin, W. R., Eades, C. G.: The action of tryptamine on the dog spinal cord and its relationship to the agonistic actions of LSD-like psychotogens. Psychopharmacologia (Berl.) 17, 242–257 (1970)

  9. Martin, W. R., Sloan, J. W.: Effects of infused tryptamine in man. Psychopharmacologia (Berl.) 18, 231–237 (1970)

  10. Martin, W. R., Sloan, J. W., Christian, S. T.: Brain tryptamine. Fed. Proc. 30, 271 (1971)

  11. Martin, W. R., Sloan, J. W., Christian, S. T., Clements, T. H.: Brain levels of tryptamine. Psychopharmacologia (Berl.) 24, 331–346 (1972)

  12. Portig, P. J., Vogt, M.: Release into the cerebral ventricles of substances with possible transmitter function in the caudate nucleus. J. Physiol. (Lond.) 204, 687–715 (1969)

  13. Saavedra, J. M., Axelrod, J.: A specific and sensitive enzymatic assay for tryptamine in tissues. J. Pharmacol. exp. Ther. 182, 363–369 (1972)

  14. Saavedra, J. M., Axelrod, J.: Effect of drugs on the tryptamine content of rat tissues. J. Pharmacol. exp. Ther. 185, 523–529 (1973)

  15. Sloan, J. W., Martin, W. R., Clements, T. H., Bridges, S. R., Buchwald, W. F.: Factors influencing brain and tissue levels of tryptamine; species, drugs and lesions. (In preparation)

  16. Snodgrass, S. R., Horn, A. S.: An assay procedure for tryptamine in brain and spinal cord using its [3H] dansyl derivative. J. Neurochem. 21, 687–696 (1973)

  17. Wilson, L. A., Boyle, W.: Rapid micro procedure for scanning distribution of subcellular components in density gradient fractions of a tissue homogenate. Analyt. Biochem. 35, 466–474 (1970)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Martin, W.R., Sloan, J.W., Buchwald, W.F. et al. The demonstration of tryptamine in regional perfusates of the dog brain. Psychopharmacologia 37, 189–198 (1974). https://doi.org/10.1007/BF00421532

Download citation

Key words

  • Tryptamine
  • Neurotransmission
  • Hallucinogens